PXD021710

PXD021710 is an original dataset announced via ProteomeXchange.

Title	Comparing Endocervical Mucus Proteome of Humans and Rhesus Macaques
Description	Endocervical mucus changes play a key role in regulating fertility throughout the menstrual cycle and in response to hormonal contraceptives. Non-human primates (NHP) provide the most translational animal model for reproductive tract studies, as they have hormonally-regulated menstrual cycles and mucus changes, similar to women. We used TMT labelling and LC-LC/MS to compare the proteins found in the mucus of the rhesus macaque to the mucus of the human endocervix. We found 3,048 total proteins present in both rhesus mucus and human mucus, and of these, 57% showed a similar expression pattern. An even higher similarity occurred in the top 500 most prevalent proteins, with overlap in 341 (68%) proteins. Mucin MUC5B was the most highly expressed mucin protein (top 10 expressed proteins in both) but other key proteins related to mucus structure were present in both samples. We find that the mucus proteome of the endocervical mucus is highly conserved in NHP and women. This supports use of the NHP model system for studies of the endocervix and trials of novel fertility treatments targeting the cervix.
HostingRepository	PRIDE
AnnounceDate	2021-05-21
AnnouncementXML	Submission_2021-05-20_22:17:54.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-28 06:01:20	ID requested
⏵ 1	2021-05-20 22:17:55	announced

Han L, Park D, Reddy A, Wilmarth PA, Jensen JT, Comparing endocervical mucus proteome of humans and rhesus macaques. Proteomics Clin Appl, 15(4):e2100023(2021) [pubmed]

submitter keyword: Endocervical mucus, human, primate, TMT labeling, quantitative proteomics

Dr. Leo Han
contact affiliation	Department of Obstetrics and Gynecology Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA
contact email	hanl@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021710

PRIDE project URI

• PRIDE
  1. PXD021710
     1. Label: PRIDE project
     2. Name: Comparing Endocervical Mucus Proteome of Humans and Rhesus Macaques