PXD021681

PXD021681 is an original dataset announced via ProteomeXchange.

Title	Proteomic response to PhiX174 infection
Description	Elucidating host-bacteriophage dynamics is an important approach to elucidating bacterial survival functions and responses to infection. The model bacteriophage φX174 endemically found amongst the human intestinal microbiota and has a long history of use in bacteriophage based research, however the common laboratory host’s response to infection lacks detailed investigation. In this study we have measured host Escherichia coli C122 proteomic and transcriptomic response to φX174 infection to fill this knowledge gap. We identify differentially expressed genes and proteins during phage induced lysis. We also use mass spectrometry to identify and quantify all φX174 proteins and over 1700 E. coli proteins enabling us to comprehensively map host pathways involved in φX174 infection. Most notably, in our dual omics investigation we observe significant host responses pertaining to membrane remodeling, cellular chaperone activity, and lipoprotein processing. We also highlight host small heat-shock proteins IbpA and IbpB as having fold-change inductions comparable to that of the phage proteins. Together, this study provides foundational and the first proteomic and transcriptomic data characterizing host response to Microviridae infection.
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-08_10:32:06.026.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bradley Wright
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-25 01:35:17	ID requested
⏵ 1	2021-04-08 10:32:06	announced

Dataset with its publication pending

submitter keyword: Bacteriophage, E. coli, omics

Mark P Molloy
contact affiliation	Faculty of Medicine and Health, Northern Clinical School, The University of Sydney
contact email	m.molloy@sydney.edu.au
lab head
Bradley Wright
contact affiliation	Macquarie University
contact email	bradley.wright@students.mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021681

PRIDE project URI

• PRIDE
  1. PXD021681
     1. Label: PRIDE project
     2. Name: Proteomic response to PhiX174 infection