PXD021675 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum |
Description | Clavulanic acid (CLV) is a betalactam (BL) which inhibits betalactamases activity and is frequently administered combined with amoxicillin (AX). Both BLs can be independently involved in allergic reactions. Indeed, selective immediate allergic reactions to CLV have recently been reported in 30% of patients allergic to the AX-CLV combination. Although protein haptenation with β-lactams is considered necessary to activate the immune system, currently there are no straight-forward detection tools for the study of protein haptenation by CLV. The aim of this study was to assess the suitability of two biotinylated analogues of CLV, CLV-B or CLV-TEG-B (containing the later a hydrophilic tetraethylenglycol linker), as probes to study protein haptenation by this β-lactam. Our results show that tagged CLV keeps some of the features of CLV, as the amide binding by which they haptenate proteins and it could constitute a valuable tool to identify protein targets for haptenation by CLV with high sensitivity to get insights into the activation of the immune system by CLV as well as mechanisms involved in allergy to β-lactams. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_08:27:32.329.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Concha Gil |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | 4800 Plus MALDI TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-24 22:41:22 | ID requested | |
⏵ 1 | 2021-09-09 08:27:33 | announced | |
Publication List
Mart, í, n-Serrano Á, Gonzalez-Morena JM, Barbero N, Ariza A, S, á, nchez G, ó, mez FJ, P, é, rez-Inestrosa E, P, é, rez-Sala D, Torres MJ, Monta, ñ, ez MI, Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum: Implications in Allergy Studies. Front Pharmacol, 11():594755(2020) [pubmed] |
Keyword List
submitter keyword: Blot and biotin detection, clavulanic acid, biotin tag, biotinylation, clavulanate, haptenation |
Contact List
María Isabel Montañez Vega |
contact affiliation | Grupo de Investigación de Enfermedades Alérgica a Alérgenos y Fármacos - Unidad de Gestión clínica de Alergología,Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain |
contact email | maribelmv.uma@gmail.com |
lab head | |
Concha Gil |
contact affiliation | UMC |
contact email | cai.proteomica@pas.ucm.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021675
- Label: PRIDE project
- Name: Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum