PXD021670

PXD021670 is an original dataset announced via ProteomeXchange.

Title	Proteome Integral Solubility Alteration assay in zebrafish embryo for exploring exposure to bioactive compounds including pollutants and chemicals mixtures
Description	The One Health initiative connects human and animal health with their shared environment. The development of novel methodology to unbiased identification of mechanisms of action of chemicals and chemicals mixtures would address one of today´s challenges of environmental and human toxicology. Zebrafish embryos offer a unique opportunity to explore the proteome integral solubility alteration (PISA) assay to study the proteins that increase or decrease their solubility as protein targets of the bioactive compounds in an organism at an developmental stage. We presented here 4 different applications of the PISA method in zebrafish embryo with different types of compounds of environmental and health concern: i) endocrine disrupting chemical, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to outline the pathways compromised under exposure; ii) complex chemical mixtures composed by TCDD + alpha-endosulfan + bisphenol A (BPA) previously studied in vitro cells lines to evaluate single chemical versus mixture; iii) novel compounds from blue-green circular economy to map the organismal response, and a iv) novel drug directed to anti-cancer activity in lymphoma models to discharge side-effects at non-target organs. Our results demonstrated the unique opportunities of PISA assay in zebrafish for toxicology. We remark some differences to its application for drug target deconvolution. First, we evaluate both targets arising from an increase in solubility and in those decreasing, as both would be involved in modulation the cellular functions. Second, the method could be applicable to bioactive compound before chemical characterization or a chemical mixture of unknown stoichiometry. Third, the results from zebrafish embryo could provide a first glimpse of unspecific interactions with proteins in non-target tissues.The introduction of PISA assay in toxi- and ecotoxicoproteomics offers high-resolution and throughput in support with the 3R, and 3M principles.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_09:31:01.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Maria Carrasco del Amor
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 06:19:24	ID requested
⏵ 1	2022-02-28 09:31:02	announced

Dataset with its publication pending

submitter keyword: Proteome Integral Solubility Alteration assay, thermal proteome profiling, zebrafish embryo, chemical mixtures, drug target, biodiscovery, bioactive compound, pollutants, novel drug

Susana Cristobal
contact affiliation	Environmental Proteomics Department of Biomedical and Clinical Sciences (BKV) Division of Cell Biology (CELLB), level 13 Medical Faculty Linköping University, SE-581 85 Linköping, Sweden.
contact email	susana.cristobal@liu.se
lab head
Ana Maria Carrasco del Amor
contact affiliation	Linköping University
contact email	ana.carrasco@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021670

PRIDE project URI

• PRIDE
  1. PXD021670
     1. Label: PRIDE project
     2. Name: Proteome Integral Solubility Alteration assay in zebrafish embryo for exploring exposure to bioactive compounds including pollutants and chemicals mixtures