PXD021666

PXD021666 is an original dataset announced via ProteomeXchange.

Title	Protein expression plasticity contributes to heat and drought tolerance of date palm
Description	Climate change is increasing the frequency and intensity of warming and drought periods around the globe, currently representing a threat to many plant species. Understanding the resistance and resilience of plants to climate change is, therefore, urgently needed. As date palm (Phoenix dactylifera) evolved adaptation mechanisms to a xeric environment and is able to tolerate large diurnal and seasonal temperature fluctuations, we studied the protein expression changes in their leaves, volatile organic compound emissions, and photosynthesis in response to variable growth temperatures and soil water deprivation. Plants were grown under controlled environmental conditions under simulated Saudi Arabian summer and winter climates followed by drought stress. We show that date palm is able to counteract the harsh conditions of the Arabian Peninsula by adjusting the abundances of proteins related to the photosynthetic machinery, abiotic stress and secondary metabolism. Under summer climate and water deprivation, these adjustments included efficient protein expression response mediated by heat shock proteins and the antioxidant system to counteract reactive oxygen species formation. Proteins related to secondary metabolism were downregulated, except for the P. dactylifera isoprene synthase (PdIspS), which was strongly upregulated in response to summer climate and drought. This study reports for the first time, the identification and functional characterization of the gene encoding for PdIspS, allowing future analysis of isoprene functions in date palm under extreme environments. Overall, the current results show that protein reprogramming of date palm leaves contribute to heat and drought tolerance. We conclude that the protein plasticity of date palm is one important mechanism of molecular adaptation to remarkable environmental fluctuations.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:11:12.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Phoenix dactylifera; NCBI TaxID: 42345;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 03:11:46	ID requested
⏵ 1	2022-02-16 08:11:13	announced

Ghirardo A, Nosenko T, Kreuzwieser J, Winkler JB, Kruse J, Albert A, Merl-Pham J, Lux T, Ache P, Zimmer I, Alfarraj S, Mayer KFX, Hedrich R, Rennenberg H, Schnitzler JP, Protein expression plasticity contributes to heat and drought tolerance of date palm. Oecologia, 197(4):903-919(2021) [pubmed]

submitter keyword: abiotic stress

heat and drought

isoprene

VOC emissions

proteomics

photosynthesis

Phoenix dactylifera

Jörg-Peter Schnitzler
contact affiliation	Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München
contact email	jp.schnitzler@helmholtz-muenchen.de
lab head
Juliane Merl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021666

PRIDE project URI

• PRIDE
  1. PXD021666
     1. Label: PRIDE project
     2. Name: Protein expression plasticity contributes to heat and drought tolerance of date palm