PXD021660

PXD021660 is an original dataset announced via ProteomeXchange.

Title	Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach
Description	As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. Using large-scale peptidomic analysis of peptide extracts from 4 different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) and a potential (novel) neuropeptides were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of 2 conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide PaOGS36577. Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach.
HostingRepository	PRIDE
AnnounceDate	2020-11-13
AnnouncementXML	Submission_2020-11-13_07:06:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HuanChao Zeng
SpeciesList	scientific name: Periplaneta americana; NCBI TaxID: 6978;
ModificationList	monohydroxylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 01:23:31	ID requested
⏵ 1	2020-11-13 07:06:04	announced

Zeng H, Qin Y, Du E, Wei Q, Li Y, Huang D, Wang G, Veenstra JA, Li S, Li N, Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach. J Proteome Res, 20(2):1217-1228(2021) [pubmed]

submitter keyword: Periplaneta americana, LCMS,Peptidomics, Genomics, Neuropeptides

Sheng Li
contact affiliation	Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University GuangZhou China
contact email	lisheng@scnu.edu.cn
lab head
HuanChao Zeng
contact affiliation	South China Normal University
contact email	851282992@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021660
     1. Label: PRIDE project
     2. Name: Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach