PXD021660 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach |
Description | As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. Using large-scale peptidomic analysis of peptide extracts from 4 different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) and a potential (novel) neuropeptides were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of 2 conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide PaOGS36577. Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-13 |
AnnouncementXML | Submission_2020-11-13_07:06:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | HuanChao Zeng |
SpeciesList | scientific name: Periplaneta americana; NCBI TaxID: 6978; |
ModificationList | monohydroxylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-24 01:23:31 | ID requested | |
⏵ 1 | 2020-11-13 07:06:04 | announced | |
Publication List
Zeng H, Qin Y, Du E, Wei Q, Li Y, Huang D, Wang G, Veenstra JA, Li S, Li N, Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach. J Proteome Res, 20(2):1217-1228(2021) [pubmed] |
Keyword List
submitter keyword: Periplaneta americana, LCMS,Peptidomics, Genomics, Neuropeptides |
Contact List
Sheng Li |
contact affiliation | Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University GuangZhou China |
contact email | lisheng@scnu.edu.cn |
lab head | |
HuanChao Zeng |
contact affiliation | South China Normal University |
contact email | 851282992@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021660
- Label: PRIDE project
- Name: Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach