PXD021652

PXD021652 is an original dataset announced via ProteomeXchange.

Title	Skeletal Muscle Proteomics of High Functioning Octogenarians
Description	Skeletal muscle mass and function decline more precipitously after the age 75 y. To provide insights into biologically relevant mechanisms for preserving muscle mass and physical function in advanced age, a battery of physical function tests, muscle cross-sectional area by MRI and a vastus lateralis muscle biopsy were performed in 15 octogenarian world class track and field masters athletes (MA) and 14 non-athlete age- and sex-matched controls (NA). Muscle samples were analyzed using liquid-chromatography mass spectrometry to generate proteomics data, by histochemistry to identify respiratory compromised muscle fibers, and by quantitative real-time polymerase chain reaction to estimate mtDNA copy number. Physical function and muscle cross-sectional area were higher in MA than NA. Of nearly 6,000 proteins identified and quantified in muscle biopsy specimens, ~800 were differentially represented in MA versus NA. Most of the differentially represented proteins related to mitochondrial structure and function and were overrepresented in muscle of MA versus controls, including TCA cycle, respiratory electron transport capacity (ETC), cristae formation, sirtuins, and 8 mtDNA-encoded proteins. On the contrary, proteins from the spliceosome complex and nuclear pore were downregulated in MA. Consistent with proteomics, MA had fewer respiratory compromised fibers, higher mtDNA copy number, and an increased protein ratio of the cristae-bound ETC subunits relative to the outer mitochondrial membrane protein voltage dependent anion channel. In conclusion, high physical function in advanced age is associated with preservation of mitochondrial health and function, with underrepresentation of proteins that process pre-RNA and determine splicing variants, and underrepresentation of nuclear pore proteins.
HostingRepository	MassIVE
AnnounceDate	2022-04-22
AnnouncementXML	Submission_2022-04-22_06:27:19.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ceereena Ubaida-Mohien
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 20:12:20	ID requested
⏵ 1	2022-04-22 06:27:20	announced

Ubaida-Mohien C, Spendiff S, Lyashkov A, Moaddel R, MacMillan NJ, Filion ME, Morais JA, Taivassalo T, Ferrucci L, Hepple RT, Unbiased proteomics, histochemistry, and mitochondrial DNA copy number reveal better mitochondrial health in muscle of high-functioning octogenarians. Elife, 11():(2022) [pubmed]

submitter keyword: Octogenarians, Master Athlete, Mitochondria, Spliceosome, Skeletal Muscle, Aging, Exercise, Physical Activity, Nuclear pore

Russell T. Hepple
contact affiliation	Department of Physical Therapy and Department of Physiology & Functional Genomics, University of Florida
contact email	rthepple@ufl.edu
lab head
Luigi Ferrucci
contact affiliation	National Institute on Aging
contact email	ferruccilu@grc.nia.nih.gov
lab head
Ceereena Ubaida-Mohien
contact affiliation	National Institute on Aging
contact email	ceereena.ubaida-mohien@nih.gov
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086195/