PXD021644

PXD021644 is an original dataset announced via ProteomeXchange.

Title	Juvenile hormone membrane signaling phosphorylates USP to potentiate 20-hydroxyecdysone action and Yorkie activity in Drosophila
Description	Juvenile hormone (JH) antagonizes 20-hydroxyecdysone (20E) signaling mainly through the intracellular receptors Met and Gce in Drosophila. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Importantly, JH was identified to phosphorylate USP at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated PKC to phosphorylate USP at Ser35. The USPS35A mutant, in which Ser35 was replaced with Ala35 by genome editing, showed attenuated 20E signaling and Yorkie activity, resulting in delayed developmental timing and reduced body size, respectively. Moreover, genetic interaction experiments confirmed that JH lied upstream of PKC-mediated USP phosphorylation at Ser35. This study propose a novel model of JH membrane signaling that exhibits crosstalk with different pathways simultaneously to regulate distinct developmental processes.
HostingRepository	iProX
AnnounceDate	2020-09-23
AnnouncementXML	Submission_2023-08-24_21:11:47.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suning Liu
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 03:26:30	ID requested
1	2020-09-23 03:37:12	announced
⏵ 2	2023-08-24 21:11:48	announced	2023-08-25: Update publication information.

Gao Y, Liu S, Jia Q, Wu L, Yuan D, Li EY, Feng Q, Wang G, Palli SR, Wang J, Li S, Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila. Sci Bull (Beijing), 67(2):186-197(2022) [pubmed]

submitter keyword: Juvenile hormone, Receptor tyrosine kinase, PKC, Phosphoproteomics, USP, 20-hydroxyecdysone action, Yorkie activity

Sheng Li
contact affiliation	South China Normal University
contact email	lisheng@scnu.edu.cn
lab head
Suning Liu
contact affiliation	South China Normal University
contact email	liusuning@m.scnu.edu.cn
dataset submitter

iProX dataset URI