PXD021643

PXD021643 is an original dataset announced via ProteomeXchange.

Title	Identifying the impact of cadmium stress on the pneumococcal metalloproteome
Description	The toxicity of the heavy metal cadmium is well established. However, the molecular basis for how this toxicity perturbs cellular viability is still not well understood. This project investigates the cellular and molecular impacts of cadmium stress in the bacterial pathogen, Streptococcus pneumoniae. We find that cadmium depletes cellular manganese and zinc stores, leading to disruption of metalloproteins and the corresponding biochemical pathways. Furthermore, the over-accumulation of cadmium within the cells appears to facilitate mismetallation events, whereby native metal cofactors are displaced by cadmium, leading to reduced or abrogated enzymatic activity. These metalloproteomic analyses have been conducted in conjunction with transcriptomics and metabolomics to elucidate the global impacts of cadmium stress within a prokaryotic organism.
HostingRepository	PRIDE
AnnounceDate	2021-06-25
AnnouncementXML	Submission_2021-06-24_22:58:17.368.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021643
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Neville
SpeciesList	scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 02:28:20	ID requested
1	2021-06-24 19:32:14	announced
⏵ 2	2021-06-24 22:58:18	announced	2021-06-25: Updated project metadata.

10.1038/S42003-020-01417-Y;

Neville SL, Eijkelkamp BA, Lothian A, Paton JC, Roberts BR, Rosch JW, McDevitt CA, Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae. Commun Biol, 3(1):694(2020) [pubmed]

submitter keyword: cadmium, metal ion homeostasis, mismetallation, metalloproteomics,Streptococcus pneumoniae

Christopher A. McDevitt
contact affiliation	The University of Melbourne
contact email	christopher.mcdevitt@unimelb.edu.au
lab head
Stephanie Neville
contact affiliation	The University of Melbourne
contact email	stephanie.neville@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD021643

PRIDE project URI

• PRIDE
  1. PXD021643
     1. Label: PRIDE project
     2. Name: Identifying the impact of cadmium stress on the pneumococcal metalloproteome