PXD021621

PXD021621 is an original dataset announced via ProteomeXchange.

Title	Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Description	Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single residue resolution. In the present study, FPOP analysis was applied to study the hemoglobin (Hb) – haptoglobin (Hp) complex allowing identification of respective regions altered upon the complex formation. The footprinting using a timsTOF Pro mass spectrometer allowed to obtain structural information for 84 and 76 residues in Hp and Hb, respectively, including statistically significant differences in the modification extent below 0.3 %. The most affected residues upon the complex formation were Met76 and Tyr140 in Hbα, and Tyr280 and Trp284 in Hpβ. The data allowed determination of amino acids directly involved in Hb – Hp interactions and those located outside of the interaction interface yet affected by the complex formation. Also, previously modeled interaction between Hb βTrp37 and Hp βPhe292 was not confirmed by our data.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_03:55:29.162.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dmitry Loginov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-22 06:29:24	ID requested
1	2021-12-30 14:36:16	announced
⏵ 2	2022-02-14 03:55:30	announced	2022-02-14: Updated project metadata.

Loginov DS, Fiala J, Brechlin P, Kruppa G, Novak P, Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex. Biochim Biophys Acta Proteins Proteom, 1870(2):140735(2022) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: footprinting, hemoglobin, haptoglobin,FPOP, timsTOF Pro

Petr Novak
contact affiliation	Institute of Microbiology of the CAS
contact email	pnovak@biomed.cas.cz
lab head
Dmitry Loginov
contact affiliation	BioCeV - Institute of Microbiology of the CAS
contact email	dloginov@jcu.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021621
     1. Label: PRIDE project
     2. Name: Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex