PXD021600

PXD021600 is an original dataset announced via ProteomeXchange.

Title	Anammox enrichment culture glycoproteomics
Description	The physiology of the planctomycetal anammox bacteria makes them particularly special because they share features with all three domains of life. Anammox bacteria have been reported recently to produce surface-layer proteins, which represent the outermost layer and provide structure, shape and protection under extreme conditions. Furthermore, we report on the unique cell surface-layer glycosylation of the anammox bacterium Ca. Kuenenia stuttgartiensis as revealed by a newly established glycoproteomics approach. This approach enables untargeted exploration of prokaryotic protein glycosylation from (high-resolution) shotgun proteomics data directly.
HostingRepository	PRIDE
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2021-08-06_01:09:47.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Pabst
SpeciesList	scientific name: Candidatus Brocadia sapporoensis; NCBI TaxID: 392547; scientific name: Candidatus Kuenenia stuttgartiensis; NCBI TaxID: 174633;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-21 22:55:35	ID requested
1	2021-08-05 02:54:49	announced
⏵ 2	2021-08-06 01:09:48	announced	2021-08-06: Updated project metadata.

10.1038/S41396-021-01073-Y;

Pabst M, Grouzdev DS, Lawson CE, Kleikamp HBC, de Ram C, Louwen R, Lin YM, L, ü, cker S, van Loosdrecht MCM, Laureni M, A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium. ISME J, 16(2):346-357(2022) [pubmed]

submitter keyword: mass binning,Microbial glycoproteomics, anammox bacteria, metaproteomics

Dr. Martin Pabst
contact affiliation	Department of Biotechnology, Delft University of Technology, The Netherlands.
contact email	m.pabst@tudelft.nl
lab head
Martin Pabst
contact affiliation	TUD
contact email	m.pabst@tudelft.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD021600

PRIDE project URI

• PRIDE
  1. PXD021600
     1. Label: PRIDE project
     2. Name: Anammox enrichment culture glycoproteomics