PXD021585

PXD021585 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of virion and genomic fibre of Mimivirus reunion
Description	Mimivirus is the prototype of the Mimiviridae family of giant dsDNA viruses, initially isolated in Acanthamoeba. Little is known about the organization of the viral genome inside the membrane limited nucleoid and whether unpacking or other rearrangements are required prior to early transcription and replication. Here we used Mimivirus reunion (an isolate from La Réunion Island) and showed that in vitro opening of its large icosahedral capsid leads to the release of electron dense, 30 nm diameter rod-shaped objects that appear to be expelled and unwinding from the particles. We developed a purification procedure and performed a mass spectrometry-based characterization of the protein content of the genomic fibre, as well as total virion.
HostingRepository	PRIDE
AnnounceDate	2022-07-29
AnnouncementXML	Submission_2022-07-29_08:51:04.036.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021585
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Acanthamoeba castellanii mimivirus; NCBI TaxID: 1899318;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-21 01:23:19	ID requested
⏵ 1	2022-07-29 08:51:04	announced

Villalta A, Schmitt A, Estrozi LF, Quemin ERJ, Alempic JM, Lartigue A, Pra, ž, á, k V, Belmudes L, Vasishtan D, Colmant AMG, Honor, é FA, Cout, é Y, Gr, ü, newald K, Abergel C, The giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield. Elife, 11():(2022) [pubmed]

submitter keyword: Mimivirus reunion, virion, genomic fibre, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD021585

PRIDE project URI

• PRIDE
  1. PXD021585
     1. Label: PRIDE project
     2. Name: Proteomic analysis of virion and genomic fibre of Mimivirus reunion