⮝ Full datasets listing

PXD021578

PXD021578 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of candida glabrata after Caspofungin exposure
DescriptionCaspofungin resistance is a great concern, as the echinocandin drugs are recommended as first-line therapy for invasive candidiasis. Echinocandin resistance is conferred by mutations in FKS genes. Nevertheless, some pathways which regulate cellular stress responses could be crucial for enabling the evolution and maintenance of drug resistance. In this work, the first objective was to identify resistant mechanisms by whole genome sequencing in echinocandin-resistant C. glabrata. Then, studies of the proteomic response to caspofungin exposure and the analysis of the impact of calcineurin inhibitors on susceptibility, stress tolerance, biofilm formation, and pathogenicity in Galleria mellonella were conducted. The caspofungin resistant isolate only presented mutation in the FKS gene. Based on proteomic results, we identified 21 proteins whose abundance changed in response to caspofungin exposure. Some of these proteins are involved in wall biosynthesis, pathogenesis, and response to stress. Also, we showed that antifungal drugs in combination with CaM/Cal inhibitors could be an excellent therapeutic option, proved in an in-vitro and in-vivo model. Results, that could be potentially used to improve the outcomes of fungal diseases, especially now that antifungal resistance is increasing.
HostingRepositoryPRIDE
AnnounceDate2022-05-25
AnnouncementXMLSubmission_2022-05-25_09:09:19.002.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021578
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterConcha Gil
SpeciesList scientific name: [Candida] glabrata; NCBI TaxID: 5478;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-20 23:53:31ID requested
12022-05-25 09:09:19announced
Publication List
Ceballos-Garzon A, Monteoliva L, Gil C, Alvarez-Moreno C, Vega-Vela NE, Engelthaler DM, Bowers J, Le Pape P, Parra-Giraldo CM, Genotypic, proteomic, and phenotypic approaches to decipher the response to caspofungin and calcineurin inhibitors in clinical isolates of echinocandin-resistant Candida glabrata. J Antimicrob Chemother, 77(3):585-597(2022) [pubmed]
Keyword List
submitter keyword: Candida glabrata, Caspofungin, Preteome, LC MS/MS
Contact List
Concha Gil
contact affiliationDepartamento de MicrobiologĂ­a II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact emailconchagil@ucm.es
lab head
Concha Gil
contact affiliationUMC
contact emailcai.proteomica@pas.ucm.es
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD021578
PRIDE project URI
Repository Record List
[ + ]