PXD021578

PXD021578 is an original dataset announced via ProteomeXchange.

Title	Proteome of candida glabrata after Caspofungin exposure
Description	Caspofungin resistance is a great concern, as the echinocandin drugs are recommended as first-line therapy for invasive candidiasis. Echinocandin resistance is conferred by mutations in FKS genes. Nevertheless, some pathways which regulate cellular stress responses could be crucial for enabling the evolution and maintenance of drug resistance. In this work, the first objective was to identify resistant mechanisms by whole genome sequencing in echinocandin-resistant C. glabrata. Then, studies of the proteomic response to caspofungin exposure and the analysis of the impact of calcineurin inhibitors on susceptibility, stress tolerance, biofilm formation, and pathogenicity in Galleria mellonella were conducted. The caspofungin resistant isolate only presented mutation in the FKS gene. Based on proteomic results, we identified 21 proteins whose abundance changed in response to caspofungin exposure. Some of these proteins are involved in wall biosynthesis, pathogenesis, and response to stress. Also, we showed that antifungal drugs in combination with CaM/Cal inhibitors could be an excellent therapeutic option, proved in an in-vitro and in-vivo model. Results, that could be potentially used to improve the outcomes of fungal diseases, especially now that antifungal resistance is increasing.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:09:19.002.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021578
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: [Candida] glabrata; NCBI TaxID: 5478;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-20 23:53:31	ID requested
⏵ 1	2022-05-25 09:09:19	announced

Ceballos-Garzon A, Monteoliva L, Gil C, Alvarez-Moreno C, Vega-Vela NE, Engelthaler DM, Bowers J, Le Pape P, Parra-Giraldo CM, Genotypic, proteomic, and phenotypic approaches to decipher the response to caspofungin and calcineurin inhibitors in clinical isolates of echinocandin-resistant Candida glabrata. J Antimicrob Chemother, 77(3):585-597(2022) [pubmed]

submitter keyword: Candida glabrata, Caspofungin, Preteome, LC MS/MS

Concha Gil
contact affiliation	Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact email	conchagil@ucm.es
lab head
Concha Gil
contact affiliation	UMC
contact email	cai.proteomica@pas.ucm.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021578
     1. Label: PRIDE project
     2. Name: Proteome of candida glabrata after Caspofungin exposure