PXD021577

PXD021577 is an original dataset announced via ProteomeXchange.

Title	Dietary protein in vitro digest (LC-MS)
Description	16 dietary protein preparations were digested according to a standardized invitro digestion (InfoGest) protocol, simulating gastrointestinal digestion. Resulting oligopeptide mixtures were subsequently analyzed by UPLC-MS using DDA shot-gun detection. Peak intensity data from MS1 spectra were obtained using Progenesis QI. MS1 peak data were matched and filtered based on overlap with the reference digest of Whey protein using a custom made script in R. MSMS fragmentation spectra were converted to .mgf format and de novo interpreted using SearchGUI 3.3.17 and RapidNOVOR algorithm. Raw data, peak intensity tables and de novo interpretaion data are provided.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_08:56:19.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine America
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Zea mays (Maize); NCBI TaxID: 4577; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-20 23:49:31	ID requested
⏵ 1	2021-09-09 08:56:20	announced

Jochems PGM, Keusters WR, America AHP, Rietveld PCS, Bastiaan-Net S, Ari, ë, ns RMC, Tomassen MMM, Lewis F, Li Y, Westphal KGC, Garssen J, Wichers HJ, van Bergenhenegouwen J, Masereeuw R, A combined microphysiological-computational omics approach in dietary protein evaluation. NPJ Sci Food, 4(1):22(2020) [pubmed]

submitter keyword: dietary protein

in vitro digest

LC-MS

de novo

Antoine H.P. America
contact affiliation	BU Bioscience, Wageningen Plant Research, Wageningen UR. the Netherlands
contact email	a.h.p.america@wur.nl
lab head
Antoine America
contact affiliation	Wageningen Plant Research, Wageningen UR, the Netherlands
contact email	a.h.p.america@wur.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021577

PRIDE project URI

• PRIDE
  1. PXD021577
     1. Label: PRIDE project
     2. Name: Dietary protein in vitro digest (LC-MS)