PXD021575

PXD021575 is an original dataset announced via ProteomeXchange.

Title	A proteomics-based method for identifying antigens within immune complexes
Description	A novel approach to recover and identify immune complexes (ICs) was developed using size exclusion chromatography (SEC) and affinity chromatography on immunoglobulin binding columns (HiTrap Protein G). The purification process was monitored by 1D SDS-PAGE, protein staining, Western blotting and, finally, liquid chromatography tandem mass spectrometry (LC MS/MS) was used to identify the recovered antigens. This approach was applied to serum with artificially created immune complexes (ICs) comprising vaccine antigen (influenza) and antibody, which led to recovery and identification of influenza peptides within the recovered ICs. This approach was compared with the established method for IC detection and recovery, polyethylene glycol (PEG) precipitation, followed by LC MS/MS. Both approaches successfully enabled capture, recovery and characterization of immunoglobulins and influenza antigen(s) in complex with the immunoglobulins. However, PEG precipitation has the advantage of simplicity and is more suited for large scale studies.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_02:57:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021575
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew McArdle
SpeciesList	scientific name: Alphainfluenzavirus; NCBI TaxID: 197911; scientific name: Betainfluenzavirus; NCBI TaxID: 197912; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-20 23:38:47	ID requested
⏵ 1	2021-01-04 02:57:26	announced

Menikou S, McArdle AJ, Li MS, Kaforou M, Langford PR, Levin M, A proteomics-based method for identifying antigens within immune complexes. PLoS One, 15(12):e0244157(2020) [pubmed]

submitter keyword: Immune complexes, Metaproteomics, Human, Influenza, Orbitrap

Michael Levin
contact affiliation	Section of Paediatric Infectious Disease
contact email	m.levin@imperial.ac.uk
lab head
Andrew McArdle
contact affiliation	Imperial College London
contact email	a.mcardle@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD021575

PRIDE project URI

• PRIDE
  1. PXD021575
     1. Label: PRIDE project
     2. Name: A proteomics-based method for identifying antigens within immune complexes