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PXD021553

PXD021553 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies.
DescriptionChemical cross-linking (XL) coupled to mass spectrometry (MS) has become a powerful approach to probe the structure of protein assemblies. Although most of the applications concerned purified complexes, latest developments focus on large-scale in vivo studies. Pushing in this direction, we describe a new and simplified in vivo XL-MS workflow to study the cellular interactome of living bacterial cells. It is based on in vivo labeling and involves a one-step enrichment by click-chemistry on a solid support. Our approach shows an impressive efficiency on Neisseria meningitidis, leading to the identification of about 1,800 cross-links in a single LC-MS/MS run using a benchtop high-resolution Orbitrap mass spectrometer. Highly dynamic multiprotein complexes were successfully captured and characterized in all bacterial compartments, showing the great potential and precision of our large-scale approach. Our workflow paves new avenues for the deep in vivo analysis of cellular interactomes.
HostingRepositoryPRIDE
AnnounceDate2022-02-14
AnnouncementXMLSubmission_2022-02-13_16:45:49.111.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartial Rey
SpeciesList scientific name: Neisseria meningitidis; NCBI TaxID: 487;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-18 01:36:15ID requested
12021-02-25 02:23:39announced
22022-02-13 16:45:49announced2022-02-14: Updated project metadata.
Publication List
Rey M, Dhenin J, Kong Y, Nouchikian L, Filella I, Duchateau M, Dupr, é M, Pellarin R, Dum, é, nil G, Chamot-Rooke J, Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions. Anal Chem, 93(9):4166-4174(2021) [pubmed]
Keyword List
ProteomeXchange project tag: EPIC-XS
submitter keyword: Click-chemistry, Mass Spectrometry, Structural Biology, in vivo Biochemistry,Cross-linking
Contact List
Julia Chamot-Rooke
contact affiliationMass Spectrometry for Biology Unit Department of Structural Biology and Chemistry Institut Pasteur, CNRS USR 2000 28 rue du Docteur Roux, 75015 Paris, France
contact emailjulia.chamot-rooke@pasteur.fr
lab head
Martial Rey
contact affiliationCNRS, Pasteur
contact emailmartial.rey@pasteur.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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