PXD021553

PXD021553 is an original dataset announced via ProteomeXchange.

Title	A simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies.
Description	Chemical cross-linking (XL) coupled to mass spectrometry (MS) has become a powerful approach to probe the structure of protein assemblies. Although most of the applications concerned purified complexes, latest developments focus on large-scale in vivo studies. Pushing in this direction, we describe a new and simplified in vivo XL-MS workflow to study the cellular interactome of living bacterial cells. It is based on in vivo labeling and involves a one-step enrichment by click-chemistry on a solid support. Our approach shows an impressive efficiency on Neisseria meningitidis, leading to the identification of about 1,800 cross-links in a single LC-MS/MS run using a benchtop high-resolution Orbitrap mass spectrometer. Highly dynamic multiprotein complexes were successfully captured and characterized in all bacterial compartments, showing the great potential and precision of our large-scale approach. Our workflow paves new avenues for the deep in vivo analysis of cellular interactomes.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-13_16:45:49.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martial Rey
SpeciesList	scientific name: Neisseria meningitidis; NCBI TaxID: 487;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-18 01:36:15	ID requested
1	2021-02-25 02:23:39	announced
⏵ 2	2022-02-13 16:45:49	announced	2022-02-14: Updated project metadata.

Rey M, Dhenin J, Kong Y, Nouchikian L, Filella I, Duchateau M, Dupr, é M, Pellarin R, Dum, é, nil G, Chamot-Rooke J, Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions. Anal Chem, 93(9):4166-4174(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: Click-chemistry, Mass Spectrometry, Structural Biology, in vivo Biochemistry,Cross-linking

Julia Chamot-Rooke
contact affiliation	Mass Spectrometry for Biology Unit Department of Structural Biology and Chemistry Institut Pasteur, CNRS USR 2000 28 rue du Docteur Roux, 75015 Paris, France
contact email	julia.chamot-rooke@pasteur.fr
lab head
Martial Rey
contact affiliation	CNRS, Pasteur
contact email	martial.rey@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD021553

PRIDE project URI

• PRIDE
  1. PXD021553
     1. Label: PRIDE project
     2. Name: A simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies.