PXD021553 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies. |
Description | Chemical cross-linking (XL) coupled to mass spectrometry (MS) has become a powerful approach to probe the structure of protein assemblies. Although most of the applications concerned purified complexes, latest developments focus on large-scale in vivo studies. Pushing in this direction, we describe a new and simplified in vivo XL-MS workflow to study the cellular interactome of living bacterial cells. It is based on in vivo labeling and involves a one-step enrichment by click-chemistry on a solid support. Our approach shows an impressive efficiency on Neisseria meningitidis, leading to the identification of about 1,800 cross-links in a single LC-MS/MS run using a benchtop high-resolution Orbitrap mass spectrometer. Highly dynamic multiprotein complexes were successfully captured and characterized in all bacterial compartments, showing the great potential and precision of our large-scale approach. Our workflow paves new avenues for the deep in vivo analysis of cellular interactomes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-14 |
AnnouncementXML | Submission_2022-02-13_16:45:49.111.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martial Rey |
SpeciesList | scientific name: Neisseria meningitidis; NCBI TaxID: 487; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-18 01:36:15 | ID requested | |
1 | 2021-02-25 02:23:39 | announced | |
⏵ 2 | 2022-02-13 16:45:49 | announced | 2022-02-14: Updated project metadata. |
Publication List
Rey M, Dhenin J, Kong Y, Nouchikian L, Filella I, Duchateau M, Dupr, é M, Pellarin R, Dum, é, nil G, Chamot-Rooke J, Cross-Linking Mass Spectrometry Platform to Characterize Proteome-Wide Protein Interactions. Anal Chem, 93(9):4166-4174(2021) [pubmed] |
Keyword List
ProteomeXchange project tag: EPIC-XS |
submitter keyword: Click-chemistry, Mass Spectrometry, Structural Biology, in vivo Biochemistry,Cross-linking |
Contact List
Julia Chamot-Rooke |
contact affiliation | Mass Spectrometry for Biology Unit Department of Structural Biology and Chemistry Institut Pasteur, CNRS USR 2000 28 rue du Docteur Roux, 75015 Paris, France |
contact email | julia.chamot-rooke@pasteur.fr |
lab head | |
Martial Rey |
contact affiliation | CNRS, Pasteur |
contact email | martial.rey@pasteur.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021553
- Label: PRIDE project
- Name: A simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies.