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PXD021550

PXD021550 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics data of mouse skeletal muscles (microgravity exposure)
DescriptionThis project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Applying proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as osteoporosis occurring on the ground by searching serum, bone and skeletal muscle (soleus, extensor digitorum longus) proteomes. The goal of this study is to get better understanding to protect the health of both astronauts and osteoporosis patients. Proteome analyses of skeletal muscles from 12 male C57BL/6J mouse (microgravity with fructo-oligosaccharide (FOS) ingestion, microgravity without FOS ingestion, artificial 1 g with FOS ingestion,and artificial 1 g without FOS ingestion, [n=3, respectively]) were carried out tryptic digestion followed by LC-MS/MS analysis (DDA). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR threshold by searching against the randomized decoy DB.
HostingRepositoryjPOST
AnnounceDate2021-07-30
AnnouncementXMLSubmission_2022-09-18_03:01:06.746.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYayoi Kimura
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListunknown modification; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; S-carbamoyl-L-cysteine; N6-carbamoyl-L-lysine; alpha-aminocarbamoylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-17 21:50:51ID requested
12021-07-29 18:08:04announced
22021-09-20 18:31:57announced2021-09-21: Updated PubMed.
32022-09-18 03:01:07announced2022-09-18: Updated FTP location.
Publication List
Ohira T, Ino Y, Kimura Y, Nakai Y, Kimura A, Kurata Y, Kagawa H, Kimura M, Egashira K, Matsuda C, Ohira Y, Furukawa S, Hirano H, Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice. NPJ Microgravity, 7(1):34(2021) [pubmed]
Keyword List
submitter keyword: Mouse, Skeletal muscle, Soleus, Extensor digitorum longus, Microgravity exposure, Kibo project, Medical Proteomics
Contact List
Hisashi Hirano
lab head
Yayoi Kimura
contact affiliationYokohama City University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000969/