PXD021536

PXD021536 is an original dataset announced via ProteomeXchange.

Title	Heat stress triggers protein secretion into the extracellular matrix of sorghum cell suspension cultures
Description	Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes. Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein expression within the sorghum secretome. Of the 265 secreted proteins identified, 100 responded to heat, with the majority (85%) possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially expressed proteins have putative functions in detoxification (36%), metabolism (32%) and protein modifications (19%), while 13% are unclassified. Extracellular proteins play a vital role in cell-cell communications during stress adaptation. Our study reveals new insights into the adaptive response of sorghum to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops.
HostingRepository	PRIDE
AnnounceDate	2020-10-29
AnnouncementXML	Submission_2020-10-29_01:17:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adrian Brown
SpeciesList	scientific name: Sorghum bicolor; NCBI TaxID: 4558;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-17 03:52:36	ID requested
⏵ 1	2020-10-29 01:17:47	announced

Ngcala MG, Goche T, Brown AP, Chivasa S, Ngara R, Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures. Proteomes, 8(4):(2020) [pubmed]

submitter keyword: sorghum, heat stress, iTRAQ

Stephen Chivasa
contact affiliation	Durham University, UK
contact email	stephen.chivasa@durham.ac.uk
lab head
Adrian Brown
contact affiliation	Durham University
contact email	a.p.brown@durham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021536
     1. Label: PRIDE project
     2. Name: Heat stress triggers protein secretion into the extracellular matrix of sorghum cell suspension cultures