PXD021516

PXD021516 is an original dataset announced via ProteomeXchange.

Title	Strucuture-function relationships of an atypical secretory kinase essential for Toxoplasma gondii invasion
Description	An essential step in active host cell invasion by the obligate intracellular apicomplexan parasites is the formation of a moving junction (MJ), which joins both plasma membranes and connects the underlying host cortical cytoskeleton with the parasite actomyosin system. Invading Toxoplasma gondii secrete RON complex proteins from rhoptry organelles into the host cell which provide the MJ components on the cytoplasmic side of the host cell membrane. We investigated the role of an essential organelle-resident RON13 kinase and implicated in phosphorylation of rhoptry content. Structural biology and biochemistry demonstrated that RON13 is an atypical kinase inserted N-terminally in the organelle membrane and processed by the aspartyl protease 3. In the absence of RON13 function rhoptry discharge is unaffected but parasites fail to invade cells in vitro and become avirulent in a mouse model. Comparative phosphoproteomics revealed RON13-dependent phosphorylation of secreted RONs involved in anchoring the MJ in the host cell cytoskeleton.
HostingRepository	PRIDE
AnnounceDate	2021-05-11
AnnouncementXML	Submission_2021-07-20_00:24:50.732.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021516
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gaelle Lentini
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-16 08:15:23	ID requested
1	2021-05-10 22:37:54	announced
⏵ 2	2021-07-20 00:24:51	announced	2021-07-20: Updated publication reference for PubMed record(s): 34145271.

Lentini G, Ben Chaabene R, Vadas O, Ramakrishnan C, Mukherjee B, Mehta V, Lunghi M, Grossmann J, Maco B, Visentin R, Hehl AB, Korkhov VM, Soldati-Favre D, Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion. Nat Commun, 12(1):3788(2021) [pubmed]

submitter keyword: Toxoplasma gondii kinase RON complex invasion

Dominique Soldati
contact affiliation	Dpt. of Microbiology and molecular medicine, Universtity of Geneva, Geneva, Switzerland
contact email	dominique.soldati-favre@unige.ch
lab head
Gaelle Lentini
contact affiliation	Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland.
contact email	gaelle.lentini@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021516
     1. Label: PRIDE project
     2. Name: Strucuture-function relationships of an atypical secretory kinase essential for Toxoplasma gondii invasion