PXD021509

PXD021509 is an original dataset announced via ProteomeXchange.

Title	High throughput microfluidic 3D biomimetic model enabling quantitative description of human breast tumor microenvironment
Description	Cancer is driven by genetic aberrations in the tumor cells and fundamental changes in the tumor microenvironment (TME). These changes offer potential targets for novel therapeutics, yet lack of in vitro 3D models recapitulating this complex microenvironment impedes such progress. Here, we generated tumor-stroma spheroids reflecting the in vivo TME of early and advanced breast tumor, using a high throughput microfluidic system. The spheroids, composed of tumor cells, fibroblasts and activated immune cells, were generated and cultivated on-chip in alginate (Alg) or alginate-alginate sulfate (Alg/Alg-S) hydrogels.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:34:42.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Gregus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-16 01:22:51	ID requested
1	2022-10-13 12:29:02	announced
⏵ 2	2023-11-14 08:34:42	announced	2023-11-14: Updated project metadata.

Berger Fridman I, Kostas J, Gregus M, Ray S, Sullivan MR, Ivanov AR, Cohen S, Konry T, High-throughput microfluidic 3D biomimetic model enabling quantitative description of the human breast tumor microenvironment. Acta Biomater, 132():473-488(2021) [pubmed]

submitter keyword: breast cancer, microfluidics,tumor microenvironment, proteomic profiling, alginate

Alexander R. Ivanov
contact affiliation	Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United States
contact email	a.ivanov@northeastern.edu
lab head
Michal Gregus
contact affiliation	Northeastern University
contact email	gregus.michal89@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD021509

PRIDE project URI

• PRIDE
  1. PXD021509
     1. Label: PRIDE project
     2. Name: High throughput microfluidic 3D biomimetic model enabling quantitative description of human breast tumor microenvironment