PXD021505

PXD021505 is an original dataset announced via ProteomeXchange.

Title	A quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis
Description	Targeted protein degradation via the ubiquitin proteasome has been established in eukaryotes, however no application has been shown in bacteria so far. In the present work, selective protein degradation is shown using hetero bifunctional drugs, bringing substrates in close proximity to the bacterial ClpC1P1P2 protein degradation complex. We performed a TMT based quantitative proteomics analysis to monitor degradation of human BRDT in Mycobacterium smegmatis. Our data shows selective degradation of BRDT when using bacterial PROTACS, indicating the first successful application of targeted protein degradation in bacteria.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_17:24:30.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Hoi
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-16 01:22:35	ID requested
⏵ 1	2022-08-11 17:24:31	announced

Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T, BacPROTACs mediate targeted protein degradation in bacteria. Cell, 185(13):2338-2353.e18(2022) [pubmed]

submitter keyword: TPD, targeted protein degradation, protease, Mycobacteria

Markus Hartl
contact affiliation	Max Perutz Labs Vienna, Mass Spectrometry Facility, Austria
contact email	markus.hartl@univie.ac.at
lab head
David Hoi
contact affiliation	IMP
contact email	david.hoi@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD021505

PRIDE project URI

• PRIDE
  1. PXD021505
     1. Label: PRIDE project
     2. Name: A quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis