PXD021488

PXD021488 is an original dataset announced via ProteomeXchange.

Title	Different protein cargo of extracellular vesicles from yeast or hyphal Candida albicans cells
Description	Extracellular vesicles (EVs) are shed from cells of almost every type of cell or organism. These nano- to micrometer scaled lipid bilayer delimited spheres shuttle a set of bioactive molecules involved in many processes including cell-cell communication, host-pathogen interactions and even the sharing of microbial community resources. On the other hand, the yeast-to hypha transition is the most studied morphology transition in C. albicans and one of the most important traits of C. albicans to invade host tissue during infection. In this context, we have undergone the isolation and protein cargo characterization of EVs secreted by both major C. albicans morphologies by means, LC- MS analysis. A total of 2546 different proteins were identified from hyphal EVs in the 3 biological replicates carried out dropping this number to only 414 proteins identified in the 3 biological replicates from yeast EVs. Comparing the identified proteins of both EVs, 241 proteins were common to both type of vesicles. In terms of biological significance, we only took into consideration proteins that were identified in at least two biological replicates with at least two peptides and a q-value< 0.01.
HostingRepository	PRIDE
AnnounceDate	2024-09-10
AnnouncementXML	Submission_2024-09-10_05:13:45.654.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021488
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-15 05:28:28	ID requested
1	2022-04-07 11:34:52	announced
⏵ 2	2024-09-10 05:13:46	announced	2024-09-10: Updated project metadata.

Mart, í, nez-L, ó, pez R, Hern, á, ez ML, Redondo E, Calvo G, Radau S, Pardo M, Gil C, Monteoliva L, Candida albicans Hyphal Extracellular Vesicles Are Different from Yeast Ones, Carrying an Active Proteasome Complex and Showing a Different Role in Host Immune Response. Microbiol Spectr, 10(3):e0069822(2022) [pubmed]

10.6019/PXD021488;

10.1128/spectrum.00698-22;

submitter keyword: hyphae, dimorphic transition,C. albicans, extracelullar vesicles, yeast, proteome

Concha Gil
contact affiliation	Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact email	conchagil@ucm.es
lab head
Concha Gil
contact affiliation	UCM
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD021488

PRIDE project URI

• PRIDE
  1. PXD021488
     1. Label: PRIDE project
     2. Name: Different protein cargo of extracellular vesicles from yeast or hyphal Candida albicans cells