PXD021460 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The temperature quickly modulates protein expression by Codon Usage in wheat filling grain |
Description | The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features which implicated in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly drive the changes in protein expression under heat stress, and their combined contribution explains 58.2% and 66.4% of protein variation in 30 and 40 °C, respectively. Of which, transcription contributes more to the alteration in protein content under 40 °C (31%) than to 30 °C (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-24 |
AnnouncementXML | Submission_2021-03-23_19:21:05.015.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bingjin Wu |
SpeciesList | scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-14 02:07:43 | ID requested | |
⏵ 1 | 2021-03-23 19:21:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Heat stress |
Post-transcriptional regulation |
Codon usage |
AAG frequency |
Wheat |
Contact List
Bingjin Wu |
contact affiliation | Northwest A&F University, Yangling, Shaanxi, 712100, China |
contact email | wbj2010@163.com |
lab head | |
Bingjin Wu |
contact affiliation | Northwest A&F University |
contact email | wubingjin2017@nwafu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021460
- Label: PRIDE project
- Name: The temperature quickly modulates protein expression by Codon Usage in wheat filling grain