PXD021455 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Disclosing proteins in cork oak plants associated with the immune response to P. cinnamomi inoculation in a long-term assay |
Description | The pathological interaction between oak trees and Phytophthora cinnamomi has implications in the cork oak decline observed over the last decades in the Iberian Peninsula. During host colonization, the phytopathogen secretes effector molecules like elicitins to increase disease effectiveness. The objective of this study was to unravel the proteome changes associated to with the cork oak immune response triggered by P. cinnamomi inoculation in a long-term assay, through SWATH-MS quantitative proteomics performed in the oak leaves. Using the Arabidopis thaliana proteome database as a reference, 424 proteins have been confidently quantified in cork oak leaves, of which 80 proteins showed a p-value below 0.05 or a fold-change greater than 2 or less than 0.5 in their levels between control and inoculated samples being considered as altered. The inoculation of cork oak roots with P. cinnamomi increased the levels of proteins associated with protein-DNA complex assembly, lipid oxidation, response to endoplasmic reticulum stress, and pyridine-containing compound metabolic process in the leaves. In opposition, several proteins associated with cellular metabolic compound salvage and monosaccharide catabolic process had significantly decreased abundances. The most significant abundance variations were observed for the Ribulose 1,5-Bisphosphate Carboxylase small subunit (RBCS1A), Heat Shock protein 90-1 (Hsp90-1), Lipoxygenase 2 (LOX2) and Histone superfamily protein H3.3 (A8MRLO/At4G40030) revealing a pertinent role for these proteins in the host-pathogen interaction mechanism. This work represents the first SWATH-MS analysis performed in cork oak plants inoculated with P. cinnamomi and highlights host proteins that have a relevant action in the homeostatic states that emerge from the interaction between the oomycete and the host in the long term and in a distal organ. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-04 |
AnnouncementXML | Submission_2021-01-04_06:41:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ana Cristina Coelho |
SpeciesList | scientific name: Quercus suber; NCBI TaxID: 58331; |
ModificationList | S-carboxamidoethyl-L-cysteine |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-14 02:07:15 | ID requested | |
⏵ 1 | 2021-01-04 06:41:31 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cork oak plant, Quercus suber, P. cinnamomi, SWATH-MS |
Contact List
Ana Cristina Hurtado de Matos Coelho |
contact affiliation | Center for Electronic, Optoelectronic and Telecommunications (CEOT), Universidade do Algarve, Faro, Portugal |
contact email | acoelho@ualg.pt |
lab head | |
Ana Cristina Coelho |
contact affiliation | University of Algarve, 8005-139 Faro, Portugal CEOT-Center for Electronic, Optoelectronic and Telecommunications, University of Algarve, 8005-139 Faro, Portugal. |
contact email | acoelho@ualg.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021455
- Label: PRIDE project
- Name: Disclosing proteins in cork oak plants associated with the immune response to P. cinnamomi inoculation in a long-term assay