PXD021455

PXD021455 is an original dataset announced via ProteomeXchange.

Title	Disclosing proteins in cork oak plants associated with the immune response to P. cinnamomi inoculation in a long-term assay
Description	The pathological interaction between oak trees and Phytophthora cinnamomi has implications in the cork oak decline observed over the last decades in the Iberian Peninsula. During host colonization, the phytopathogen secretes effector molecules like elicitins to increase disease effectiveness. The objective of this study was to unravel the proteome changes associated to with the cork oak immune response triggered by P. cinnamomi inoculation in a long-term assay, through SWATH-MS quantitative proteomics performed in the oak leaves. Using the Arabidopis thaliana proteome database as a reference, 424 proteins have been confidently quantified in cork oak leaves, of which 80 proteins showed a p-value below 0.05 or a fold-change greater than 2 or less than 0.5 in their levels between control and inoculated samples being considered as altered. The inoculation of cork oak roots with P. cinnamomi increased the levels of proteins associated with protein-DNA complex assembly, lipid oxidation, response to endoplasmic reticulum stress, and pyridine-containing compound metabolic process in the leaves. In opposition, several proteins associated with cellular metabolic compound salvage and monosaccharide catabolic process had significantly decreased abundances. The most significant abundance variations were observed for the Ribulose 1,5-Bisphosphate Carboxylase small subunit (RBCS1A), Heat Shock protein 90-1 (Hsp90-1), Lipoxygenase 2 (LOX2) and Histone superfamily protein H3.3 (A8MRLO/At4G40030) revealing a pertinent role for these proteins in the host-pathogen interaction mechanism. This work represents the first SWATH-MS analysis performed in cork oak plants inoculated with P. cinnamomi and highlights host proteins that have a relevant action in the homeostatic states that emerge from the interaction between the oomycete and the host in the long term and in a distal organ.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_06:41:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Cristina Coelho
SpeciesList	scientific name: Quercus suber; NCBI TaxID: 58331;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-14 02:07:15	ID requested
⏵ 1	2021-01-04 06:41:31	announced

Dataset with its publication pending

submitter keyword: Cork oak plant, Quercus suber, P. cinnamomi, SWATH-MS

Ana Cristina Hurtado de Matos Coelho
contact affiliation	Center for Electronic, Optoelectronic and Telecommunications (CEOT), Universidade do Algarve, Faro, Portugal
contact email	acoelho@ualg.pt
lab head
Ana Cristina Coelho
contact affiliation	University of Algarve, 8005-139 Faro, Portugal CEOT-Center for Electronic, Optoelectronic and Telecommunications, University of Algarve, 8005-139 Faro, Portugal.
contact email	acoelho@ualg.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021455
     1. Label: PRIDE project
     2. Name: Disclosing proteins in cork oak plants associated with the immune response to P. cinnamomi inoculation in a long-term assay