PXD021442

PXD021442 is an original dataset announced via ProteomeXchange.

Title	Alterations of proteomic profiling revealed the molecular mechanisms of aristolochic acids toxicity
Description	Aristolochic acids (AAs), nephrotoxic components of herbs, has been previously demonstrated causing DNA damage by forming DNA-AAs adducts. However, the alterations of kidney proteome profiles underlying AAs nephrotoxicity remain elusive. In the present study, the proteomics analysis of the kidney and liver tissues from I/R rats after AAs treatment was performed by a shotgun proteomic approach coupled with LC-MS/MS technology. A total of 2061 and 2405 proteins were identified and quantified in kidney and liver, respectively. 112 and 95 proteins were significantly dysregulated in kidney and liver in comparison between AAs verse I/R. Based on the relative proteome quantification results, six differentially expressed proteins were selected as the candidate biomarkers of AAN. Pathway enrichment analysis results were variant in kidney and liver. Enrichment analysis of metabolic pathways showed that inflammation and cell apoptosis were common causes of AA toxicity. The effect of AAs on the biological process of the liver was less than that on the kidney, which indicated that AAs was more toxic to the kidney and caused aristolochic acid nephropathy (AAN).
HostingRepository	iProX
AnnounceDate	2021-04-15
AnnouncementXML	Submission_2021-04-14_23:30:42.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Dong
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	methionine oxidation with neutral loss of 64 Da
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-13 22:42:56	ID requested
1	2020-09-13 22:46:24	announced
⏵ 2	2021-04-14 23:30:43	announced	2021-04-15: update information.

Dataset with its publication pending

submitter keyword: aristolochic acids, shotgun proteomics, nephropathy

Jingjing Liu
contact affiliation	Nanjing University of Aeronautics and Astronautics
contact email	jjliu@nuaa.edu.cn
lab head
Wei Dong
contact affiliation	Nanjing University of Aeronautics and Astronautics
contact email	weidong@nuaa.edu.cn
dataset submitter

iProX dataset URI