PXD021441
PXD021441 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analyses of plasma-derived exosomes in IgG4-RD and its potential role in B cell differentiation and tissue damage |
Description | Objective: This study aimed to investigate plasma exosome profiling of IgG4-RD and its potential role in B cell differentiation and tissue damage. Methods: One hundred untreated IgG4-RD patients and 135 sex, age matched healthy controls (HCs) were enrolled in this study. A combination of liquid chromatography-tandem mass spectroscopy (LC-MS/MS) and TMT label quantitation was used to detect exosome and B cell profiling. To validate differentially expressed proteins, western blot and ELISA were performed. RT-qPCR was used to detect gene expression after exosome stimulation. Flowcytometry was used to measure B cell activation, apoptosis, differentiation and reactive oxygen species (ROS) expression. Besides, correlation analysis between differentially expressed complement protein and laboratory parameters was also performed. Results: In total, 82 proteins were upregulated and 96 proteins were downregulated in exosome of IgG4-RD patients compared with HCs. Pathway analysis revealed that complement cascade pathway in exosome of IgG4-RD was activated, together with reduced C3 complement and C5 complement. Validation by ELISA and WB, exosome C3 and C5 was decrease in IgG4-RD compared with HCs. Stimulation of B cells with exosome, naïve B cells decreased, while memory B cells and plasmablast were increased together with increased CYCS with activation of downstream complement system. ROS was increased in B cells of IgG4-RD compared with HC. In affected submandibular glands, BCR signaling pathway was activated and exosome may potentially participate in tissue damage. Conclusion: There were 82 differentially expressed upregulated proteins and 96 downregulated proteins in IgG4-RD exosome compared with HCs. Differentially expressed proteins were enriched in exosome, complement function and immune response indicating complement activation in exosome may be related to IgG4-RD. Reduced C3 and C5 expression in exosome of IgG4-RD may indicate the disease severity. Our research suggests that IgG4-RD exosomes can participate in the differentiation B cells into plasmablast, activate the B cell auto-oxidative damage pathway and potentially participate in the pathogenesis of IgG4-RD. |
HostingRepository | iProX |
AnnounceDate | 2020-09-14 |
AnnouncementXML | Submission_2023-08-24_21:12:35.068.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shengyu Zhang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-09-13 19:49:30 | ID requested | |
1 | 2020-09-13 19:51:23 | announced | |
⏵ 2 | 2023-08-24 21:12:35 | announced | 2023-08-25: Update publication information. |
Publication List
Zhang P, Zhang Y, Pan M, Liu Z, Li J, Peng L, Zhou J, Hu C, Liu S, Zeng X, Ge W, Zhang W, Proteomic analyses of plasma-derived exosomes in immunoglobulin (Ig) G4-related disease and their potential roles in B cell differentiation and tissue damage. J Autoimmun, 122():102650(2021) [pubmed] |
Keyword List
submitter keyword: Proteomic, IgG4-RD, exosomes, B cell |
Contact List
Wei Ge | |
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contact affiliation | Institute of Basic Medical Sciences Chinese Academy of Medical Sciences |
contact email | wei.ge@chem.ox.ac.uk |
lab head | |
Shengyu Zhang | |
contact affiliation | Institute of Basic Medical Sciences Chinese Academy of Medical Sciences |
contact email | zhendezy@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |