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PXD021441

PXD021441 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analyses of plasma-derived exosomes in IgG4-RD and its potential role in B cell differentiation and tissue damage
DescriptionObjective: This study aimed to investigate plasma exosome profiling of IgG4-RD and its potential role in B cell differentiation and tissue damage. Methods: One hundred untreated IgG4-RD patients and 135 sex, age matched healthy controls (HCs) were enrolled in this study. A combination of liquid chromatography-tandem mass spectroscopy (LC-MS/MS) and TMT label quantitation was used to detect exosome and B cell profiling. To validate differentially expressed proteins, western blot and ELISA were performed. RT-qPCR was used to detect gene expression after exosome stimulation. Flowcytometry was used to measure B cell activation, apoptosis, differentiation and reactive oxygen species (ROS) expression. Besides, correlation analysis between differentially expressed complement protein and laboratory parameters was also performed. Results: In total, 82 proteins were upregulated and 96 proteins were downregulated in exosome of IgG4-RD patients compared with HCs. Pathway analysis revealed that complement cascade pathway in exosome of IgG4-RD was activated, together with reduced C3 complement and C5 complement. Validation by ELISA and WB, exosome C3 and C5 was decrease in IgG4-RD compared with HCs. Stimulation of B cells with exosome, naïve B cells decreased, while memory B cells and plasmablast were increased together with increased CYCS with activation of downstream complement system. ROS was increased in B cells of IgG4-RD compared with HC. In affected submandibular glands, BCR signaling pathway was activated and exosome may potentially participate in tissue damage. Conclusion: There were 82 differentially expressed upregulated proteins and 96 downregulated proteins in IgG4-RD exosome compared with HCs. Differentially expressed proteins were enriched in exosome, complement function and immune response indicating complement activation in exosome may be related to IgG4-RD. Reduced C3 and C5 expression in exosome of IgG4-RD may indicate the disease severity. Our research suggests that IgG4-RD exosomes can participate in the differentiation B cells into plasmablast, activate the B cell auto-oxidative damage pathway and potentially participate in the pathogenesis of IgG4-RD.
HostingRepositoryiProX
AnnounceDate2020-09-14
AnnouncementXMLSubmission_2023-08-24_21:12:35.068.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShengyu Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-13 19:49:30ID requested
12020-09-13 19:51:23announced
22023-08-24 21:12:35announced2023-08-25: Update publication information.
Publication List
Zhang P, Zhang Y, Pan M, Liu Z, Li J, Peng L, Zhou J, Hu C, Liu S, Zeng X, Ge W, Zhang W, Proteomic analyses of plasma-derived exosomes in immunoglobulin (Ig) G4-related disease and their potential roles in B cell differentiation and tissue damage. J Autoimmun, 122():102650(2021) [pubmed]
Keyword List
submitter keyword: Proteomic, IgG4-RD, exosomes, B cell
Contact List
Wei Ge
contact affiliationInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact emailwei.ge@chem.ox.ac.uk
lab head
Shengyu Zhang
contact affiliationInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact emailzhendezy@163.com
dataset submitter
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