PXD021438

PXD021438 is an original dataset announced via ProteomeXchange.

Title	Identification of sulfenylation patterns in Plasmodium falciparum using a non-dimedone based probe
Description	Plasmodium falciparum causes the deadliest form of malaria. Adequate redox control is crucial for this protozoan parasite to overcome oxidative and nitrosative challenges, thus enabling its survival. Sulfenylation is an oxidative post-translational modification, which acts as a molecular on/off switch, regulating protein activity. To obtain a better understanding of which proteins are redox regulated in malaria parasites, we established an optimized affinity capture protocol coupled with mass spectrometry analysis for identification of in vivo sulfenylated proteins. The non-dimedone based probe BCN-Bio1 shows reaction rates over 100-times that of commonly used dimedone-based probes, allowing for a rapid trapping of sulfenylated proteins. Mass spectrometry analysis of BCN-Bio1 labeled proteins revealed the first insight into the Plasmodium falciparum sulfenylome, identifying 102 proteins containing 152 sulfenylation sites. Comparison with Plasmodium proteins modified by S-glutathionylation and S-nitrosation showed a high overlap, suggesting a common core of proteins undergoing redox regulation by multiple mechanisms. Furthermore, parasite proteins which were identified as targets for sulfenylation were also identified as being sulfenylated in other organisms, especially proteins of the glycolytic cycle. This study suggests that a number of Plasmodium proteins are subject to redox regulation and it provides a basis for further investigations into the exact structural and biochemical basis of regulation, and a deeper understanding of cross-talk between post-translational modifications.
HostingRepository	MassIVE
AnnounceDate	2021-04-09
AnnouncementXML	Submission_2021-04-09_11:50:57.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvador Martínez de Bartolomé
SpeciesList	scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833;
ModificationList	Oxidation+NEM; unknown modification
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-11 16:26:04	ID requested
⏵ 1	2021-04-09 11:50:58	announced

Schipper S, Wu H, Furdui CM, Poole LB, Delahunty CM, Park R, Yates JR, Becker K, Przyborski JM, Identification of sulfenylation patterns in trophozoite stage Plasmodium falciparum using a non-dimedone based probe. Mol Biochem Parasitol, 242():111362(2021) [pubmed]

submitter keyword: Sulfenylation, 9-hydroxymethylbicyclo[6.1.0]nonyne (BCN-Bio1), malaria, redox proteomics, cysteine modification, post-translational modification

Jude M. Przyborskia
contact affiliation	Department of Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen
contact email	Jude.Przyborski@ernaehrung.uni-giessen.de
lab head
Salvador Martínez de Bartolomé
contact affiliation	The Scripps Research Institute
contact email	salvador@scripps.edu
dataset submitter

MassIVE dataset URI

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