PXD021423

PXD021423 is an original dataset announced via ProteomeXchange.

Title	Global Lysine Crotonylation Alterations of Host Cell Proteins Caused by Brucella GNAT Containing Effector BspF
Description	In Brucella spp., the type IV secretion system (T4SS) is essential for bacterial intracellular survival and inhibition of the host innate immune response. The Brucella T4SS secretes 15 different effectors to escape host immunity and promote intracellular replication. Among them, BspF has a GNAT-family acetyltransferase domain, implying its acetyltransferase activity. To test this, in this study, we first tested and confirmed that BspF has de-histone crotonyltransferase activity in vitro. Unexpectedly, we also found that BspF enhances histone crotonylation of host cell proteins. We then conducted enrichment of crotonylated proteins and used LC-MS to study the crotonylation of proteins in HEK-293T cells caused BspF overexpression. A total of 5,559 crotonylation sites were identified on 1,525 different proteins, of which 331 sites on 265 proteins were significantly changed 30 h after transfection. Among these differentially modified proteins, a significant proportions were involved in immune response and defense mechanism. We propose that BspF may influence the function of these host proteins by changing the level of crotonylation, thereby promoting the intracellular propagation of Brucella.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_23:22:52.857.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinying Zhu
SpeciesList	scientific name: Brucella sp.; NCBI TaxID: 52132;
ModificationList	N6-crotonyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-11 00:26:34	ID requested
⏵ 1	2021-09-08 23:22:53	announced

Zhu J, Dong Q, Dong C, Zhang X, Zhang H, Chen Z, Effector BspF. Front Cell Infect Microbiol, 10():603457(2020) [pubmed]

submitter keyword: Brucella

T4SS

effector

BspF

lysine crotonylation

crotonyltransferase

Zeliang Chen
contact affiliation	Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Liaoning Province, Shenyang 110866, P. R. China
contact email	zeliangchen@yahoo.com
lab head
Jinying Zhu
contact affiliation	Shenyang Agricultural University
contact email	jean_zz512@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021423

PRIDE project URI

• PRIDE
  1. PXD021423
     1. Label: PRIDE project
     2. Name: Global Lysine Crotonylation Alterations of Host Cell Proteins Caused by Brucella GNAT Containing Effector BspF