PXD021422 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systematic Identification of Protein Phosphorylation-Mediated Interactions |
Description | Protein phosphorylation is a key regulatory mechanism involved in nearly every eukaryotic cellular process. Increasingly sensitive mass spectrometry approaches have identified hundreds of thousands of phosphorylation sites but the functions of a vast majority of these sites remain unknown, with less than 5% of sites currently assigned a function. To increase our understanding of functional protein phosphorylation we developed an approach for identifying the phosphorylation dependence of protein assemblies in a systematic manner. A combination of non-specific protein phosphatase treatment, size-exclusion chromatography, and mass spectrometry allowed us to identify changes in protein interactions after the removal of phosphates. With this approach we were able to identify 316 proteins involved in phosphorylation-sensitive interactions. We recovered known phosphorylation-dependent interactors such as the FACT complex and spliceosome, as well as identified novel interactions such as the tripeptidyl peptidase TPP2 and the supraspliceosome component ZRANB2. More generally, we find phosphorylation-dependent interactors to be strongly enriched for RNA-binding proteins, providing new insight into the role of phosphorylation in RNA binding. By searching directly for phosphorylated amino acid residues, we identified the likely regulatory phosphosites on ZRANB2 and FACT complex subunit SSRP1. This study provides both a method and resource for obtaining a better understanding of the role of phosphorylation in native macromolecular assemblies. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-22 |
AnnouncementXML | Submission_2021-01-24_22:34:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021422 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Brendan Floyd |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-11 00:19:51 | ID requested | |
1 | 2020-09-22 01:07:52 | announced | |
⏵ 2 | 2021-01-24 22:34:58 | announced | 2021-01-25: Updated publication reference for PubMed record(s): 33476154. |
Publication List
Floyd BM, Drew K, Marcotte EM, Systematic Identification of Protein Phosphorylation-Mediated Interactions. J Proteome Res, 20(2):1359-1370(2021) [pubmed] |
Keyword List
submitter keyword: Human, Shotgun, SEC, Complexes |
Contact List
Edward Marcotte |
contact affiliation | The University of Texas at Austin |
contact email | marcotte@austin.utexas.edu |
lab head | |
Brendan Floyd |
contact affiliation | University of Texas at Austin |
contact email | bmfloyd@utexas.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021422
- Label: PRIDE project
- Name: Systematic Identification of Protein Phosphorylation-Mediated Interactions