PXD021415

PXD021415 is an original dataset announced via ProteomeXchange.

Title	The Novel PII-Interacting Regulator PirC (Sll0944) Identifies 3-Phosphoglycerate Mutase (PGAM) as Central Control Point of Carbon Storage Metabolism in Cyanobacteria
Description	Nitrogen limitation imposes a major transition in the life-style of non-diazotrophic cyanobacteria, which is regulated via a complex interplay of regulatory factors, involving, the nitrogen-specific transcription factor NtcA and the pervasive signal processor PII. Immediately upon nitrogen-limitation, newly fixed carbon is re-directed towards glycogen synthesis. How operation of the metabolic switch for distributing fixed carbon to either glycogen or cellular building blocks was poorly understood. Here we identify from Synechocystis sp. PCC 6803 a novel PII interactor, PirC, (Sll0944) that controls 3-phosphoglycerate mutase (PGAM), the enzyme that deviates newly fixed CO2 towards lower glycolysis. PirC acts as competitive inhibitor of PGAM and this interaction is tuned by PII/2-oxoglutarate. High oxoglutarate release PirC from PII-complex to inhibit PGAM. Accordingly, PirC deficient mutant, as compared to the wild-type, shows strongly reduced glycogen levels upon nitrogen deprivation whereas polyhydroxybutyrate granules are over-accumulated. Metabolome analysis revealed an imbalance in 3-phosphoglycerate to pyruvate levels in the PirC mutant, conforming that PirC controls the carbon flux in cyanobacteria via mutually exclusive interaction with either PII or PGAM.
HostingRepository	PRIDE
AnnounceDate	2020-12-17
AnnouncementXML	Submission_2021-07-13_21:56:47.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-10 23:17:30	ID requested
1	2020-12-17 03:15:23	announced
⏵ 2	2021-07-13 21:56:48	announced	2021-07-14: Updated publication reference for PubMed record(s): 33526690.

Orthwein T, Scholl J, Sp, ä, t P, Lucius S, Koch M, Macek B, Hagemann M, Forchhammer K, -interactor PirC identifies phosphoglycerate mutase as key control point of carbon storage metabolism in cyanobacteria. Proc Natl Acad Sci U S A, 118(6):(2021) [pubmed]

submitter keyword: Cyanobacteria, Synechocystis sp. PCC 6803, PII, PirC (Sll0944), 3-Phosphoglycerate Mutase

Karl Forchhammer
contact affiliation	Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany
contact email	karl.forchhammer@uni-tuebingen.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Quantitative Proteomics
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021415
     1. Label: PRIDE project
     2. Name: The Novel PII-Interacting Regulator PirC (Sll0944) Identifies 3-Phosphoglycerate Mutase (PGAM) as Central Control Point of Carbon Storage Metabolism in Cyanobacteria