PXD021414

PXD021414 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Extracellular Vesicles Produced by Granulicatella adiacens, which causes Infective Endocarditis
Description	Granulicatella adiacens is a non-motile, non-spore-forming, facultatively anaerobic Gram-positive coccus. It is part of the normal oral flora but cause serious infections such as infective endocarditis. Many bacterial species produce extracellular vesicles (EVs) as part of normal physiology, but also use it as a virulence strategy. In this study, it was hypothesized that G. adiacens produces EVs that possibly help the species in virulence. Therefore, the objectives were to isolate and characterize EVs produced by these species and to investigate their immune-stimulatory effects. The reference strain G. adiacens CCUG 27809 was cultured on chocolate blood agar for 2 days. From subsequent broth cultures, the EVs were isolated using differential centrifugation and filtration protocol and then observed using scanning electron microscopy. Proteins in the vesicle preparations were identified by nano LC-ESI-MS/MS. The EVs proteomes were analyzed and characterized using different bioinformatics tools. The immune-stimulatory effect of the EVs was studied via ELISA quantification of IL-8, IL-1β and CCL5, major proinflammatory cytokines, produced from stimulated human PBMCs. It was revealed that both G. adiacens and G. elegans produced EVs, ranging in diameter from 30 to 250 nm. Overall, G. adiacens EVs contained 112 proteins. The proteome consist of several ribosomal proteins, DNA associated proteins, binding proteins, and metabolic enzymes. It was also shown that these EVs carry putative virulence factors including moonlighting proteins. These EVs were able to induce the production of IL-8, IL-1β and CCL5 from human PBMCs. The diversity in EVs content indicates that these vesicles may have possible roles in bacterial survival, invasion, host immune modulation as well as infection. Further characterization of the composition and immunogenicity of G. adiacens EVs may provide new insights into virulence mechanisms of this bacterial species.
HostingRepository	PRIDE
AnnounceDate	2020-11-05
AnnouncementXML	Submission_2020-11-04_23:25:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maribasappa Karched
SpeciesList	scientific name: Granulicatella adiacens ATCC 49175; NCBI TaxID: 638301;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-10 23:15:16	ID requested
⏵ 1	2020-11-04 23:25:55	announced

Dataset with its publication pending

submitter keyword: Granulicatella, Extravellular vesicles, Proteomics

Maribasappa Karched
contact affiliation	Oral Microbiology research Laboratory, Faculty of Dentistry, Kuwait University, KUWAIT
contact email	maribasappa.karched@gmail.com
lab head
Maribasappa Karched
contact affiliation	Faculty of Dentistry Kuwait University
contact email	maribasappa.karched@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021414

PRIDE project URI

• PRIDE
  1. PXD021414
     1. Label: PRIDE project
     2. Name: Proteomics of Extracellular Vesicles Produced by Granulicatella adiacens, which causes Infective Endocarditis