PXD021406

PXD021406 is an original dataset announced via ProteomeXchange.

Title	N-terminomics for the identification of in-vitro substrates and cleavage site specificity of the SARS-CoV-2 main protease
Description	The genome of coronaviruses, including the SARS-CoV-2, which causes the human pandemic COVID-19, encodes for two proteases, a papain like (PL) and the so-called Main protease (Mpro), also named 3CLpro or non-structural protein 5 (NSP5). Mpro is activated by autoproteolysis, and true to its name, is the main protease responsible for cutting the viral polyprotein into functional viral proteins. Aside from this function, it has been described that 3CL proteases are also capable to process host proteins, including those involved in the host innate immune response. We performed a LC-MS based N-terminomics analysis to identify in vitro substrates of three different recombinantly expressed coronavirus main proteases (SARS-CoV, SARS-CoV-2, hCoV-NL63) using lung cell lysates as substrate pools.
HostingRepository	PRIDE
AnnounceDate	2020-11-02
AnnouncementXML	Submission_2020-11-01_22:49:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-10 07:17:01	ID requested
⏵ 1	2020-11-01 22:49:56	announced

Koudelka T, Boger J, Henkel A, Sch, ö, nherr R, Krantz S, Fuchs S, Rodr, í, guez E, Redecke L, Tholey A, N-Terminomics for the Identification of In Vitro Substrates and Cleavage Site Specificity of the SARS-CoV-2 Main Protease. Proteomics, 21(2):e2000246(2021) [pubmed]

ProteomeXchange project tag: Sars-cov-2

submitter keyword: SARS-CoV-2, SARS-CoV, Proteomics, LCMS, N-Terminomics

Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, UKSH, Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021406

PRIDE project URI

• PRIDE
  1. PXD021406
     1. Label: PRIDE project
     2. Name: N-terminomics for the identification of in-vitro substrates and cleavage site specificity of the SARS-CoV-2 main protease