PXD021402

PXD021402 is an original dataset announced via ProteomeXchange.

Title	C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected ACE2-A549 cells (Enriched - Variable search)
Description	Many events in viral infection are regulated through post-translational modification. In the case of positive-sense RNA viruses such as SARS-CoV-2 which encodes two viral proteases, proteolytic cleavage of both viral and cellular proteins is crucial for productive viral infection. In this work we study proteolysis in the context of SARS-CoV-2 infection of ACE2-A549 and Vero E6 cells, infected at a multiplicity of infection of 1, with samples harvested at 0,6,12 and 24h post-infection. Neo-N-termini and lysine residues were labelled at the protein level with TMTpro reagents prior to tryptic digestion. For unenriched samples, the trypsin-digested material was then fractionated offline, and analysed by LC-MS/MS for the study of total protein abundance over the infection timecourse. For enriched samples, unblocked neo-N-termini generated by tryptic digested were labelled with undecanal and depleted, yielding a sample enriched for blocked neo-N-termini (TMTpro, N-terminal acetylation, and pyroGlu). The enriched samples were similarly fractionated offline and analysed by LC-MS/MS. Please note this is one of several linked datasets. This dataset contains data from ACE2-A549 cells infected with SARS-CoV-2, enriched for neo-N-termini.
HostingRepository	PRIDE
AnnounceDate	2021-07-29
AnnouncementXML	Submission_2021-07-29_03:10:33.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ed Emmott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-10 07:16:29	ID requested
⏵ 1	2021-07-29 03:10:33	announced

Dataset with its publication pending

submitter keyword: SARS-CoV-2, ACE2-A549, COVID-19, N-terminomics, TMTpro

Edward Emmott
contact affiliation	Centre for Proteome Research, University of Liverpool, Liverpool, UK
contact email	e.emmott@liverpool.ac.uk
lab head
Ed Emmott
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	e.emmott@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021402
     1. Label: PRIDE project
     2. Name: C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected ACE2-A549 cells (Enriched - Variable search)