PXD021401

PXD021401 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Profiling of Cervicovaginal Fluid from Pregnant Women with Term and Preterm Birth
Description	Preterm birth (PTB) is one of major causes of perinatal mortality and neonatal morbidity, but knowledge of its complex etiology is still limited. Here we present cervicovaginal fluid (CVF) protein profiles of pregnant women who subsequently delivered at spontaneous preterm or term, aiming to identify differentially expressed CVF proteins in PTB and term birth. The CVF proteome of women who sequentially delivered at preterm and term was analyzed using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional nanoflow liquid chromatography-tandem mass spectrometry (2D-nLC-MS/MS). We compared the CVF proteome of PTB (n=5) and control subjects (term birth, n=7) using pooled control CVF (term birth, n=20) as spike-in standard. We identified 1294 CVF proteins, of which 605 were newly identified proteins. Of 990 proteins quantified in both PTB and term birth, 52 proteins were significantly up/down-regulated in PTB compared to term birth. The differentially expressed proteins were functionally associated to immune response, endopeptidase inhibitors and structural constituent of cytoskeleton. Taken together, our study provide quantitative CVF proteome profiles of pregnant women who ultimately delivered at preterm and term. These promising results could help to improve the understanding of PTB etiology and to discover biomarkers for asymptomatic PTB.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_09:23:57.373.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Youn Eun Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-10 07:16:27	ID requested
⏵ 1	2021-09-09 09:23:58	announced

Kim YE, Kim K, Oh HB, Lee SK, Kang D, Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth. Proteome Sci, 19(1):3(2021) [pubmed]

submitter keyword: cervicovaginal fluid, preterm birth, quantitative proteomics

Dukjin Kang
contact affiliation	Center for Bioanalysis, Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, Korea
contact email	djkang@kriss.re.kr
lab head
Youn Eun Kim
contact affiliation	267, Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea
contact email	youngeun@kaist.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021401
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Profiling of Cervicovaginal Fluid from Pregnant Women with Term and Preterm Birth