PXD021396 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1 |
Description | Extracellular vesicles (EVs) are secreted by myriad cells in culture and also by unicellular organisms, and their identification in mammalian fluids suggests that EV release also occurs at the organism level. However, although it is clearly important to better understand EVs' roles in organismal biology, EVs in solid tissues have received little attention. Here, we modified a protocol for EV isolation from primary neural cell culture to collect EVs from frozen whole murine and human neural tissues by serial centrifugation and purification on a sucrose gradient. Quantitative proteomics comparing brain-derived EVs from nontransgenic (NTg) and a transgenic amyotrophic lateral sclerosis (ALS) mouse model, superoxide dismutase 1 (SOD1) G93A , revealed that these EVs contain canonical exosomal markers and are enriched in synaptic and RNA-binding proteins. The compiled brain EV proteome contained numerous proteins implicated in ALS, and EVs from SOD1 G93A mice were significantly depleted in myelin-oligodendrocyte glycoprotein compared with those from NTg animals. We observed that brain- and spinal cord–derived EVs, from NTg and SOD1 G93A mice, are positive for the astrocyte marker GLAST and the synaptic marker SNAP25, whereas CD11b, a microglial marker, was largely absent. EVs from brains and spinal cords of the SOD1 G93A ALS mouse model, as well as from human SOD1 familial ALS patient spinal cord, contained abundant misfolded and nonnative disulfide-cross-linked aggregated SOD1. Our results indicate that CNS-derived EVs from an ALS animal model contain pathogenic disease-causing proteins and suggest that brain astrocytes and neurons, but not microglia, are the main EV source. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-11 |
AnnouncementXML | Submission_2020-09-10_22:42:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jenny Moon |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-10 01:38:26 | ID requested | |
⏵ 1 | 2020-09-10 22:42:18 | announced | |
Publication List
Silverman JM, Christy D, Shyu CC, Moon KM, Fernando S, Gidden Z, Cowan CM, Ban Y, Stacey RG, Grad LI, McAlary L, Mackenzie IR, Foster LJ, Cashman NR, ALS mice originate from astrocytes and neurons and carry misfolded SOD1. J Biol Chem, 294(10):3744-3759(2019) [pubmed] |
Keyword List
submitter keyword: exosome (vesicle), amyotrophic lateral sclerosis (ALS) (Lou Gehrig disease), neurodegeneration, proteomics, astrocyte, extracellular vesicles, central nervous system (CNS), secretion, protein homeostasis, mouse model |
Contact List
Neil R. Cashman |
contact affiliation | Centre for Brain Health, Dept. of Medicine, University of British Columbia, Vancouver, British Columbia V6T 1B5, Canada |
contact email | neil.cashman@vch.ca |
lab head | |
Jenny Moon |
contact affiliation | University of British Columbia |
contact email | kyungmee@mail.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021396
- Label: PRIDE project
- Name: CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1