PXD021375

PXD021375 is an original dataset announced via ProteomeXchange.

Title	Interactome of the -subunit of L-type voltage-gated calcium channels in adult cardiomyocytes
Description	In cardiomyocytes, Ca2+ influx through L-type voltage-gated calcium channels (LTCCs) following membrane depolarization regulates crucial Ca2+-dependent processes including duration and amplitude of the action potentials and excitation-contraction coupling. LTCCs are heteromultimeric proteins composed of the Cav1, Cav, Cav2 and Cav subunits. Here, using ascorbate peroxidase (APEX)-mediated proximity labeling and quantitative proteomics, we identified 61 proteins in the nano-environments of Cav2 in cardiomyocytes. These proteins are involved in diverse cellular functions such as cellular trafficking, muscular contraction, sarcomere organization and excitation-contraction coupling. Moreover, pull-down assays, co-immunoprecipitation analyses and super-resolution imaging using direct stochastic optical reconstruction microscopy (dSTORM) revealed that Cav2 interacts with the ryanodine receptor 2 (RyR2) in adult cardiomyocytes, probably coupling LTCCs and the RyR2 into a supramolecular complex at the dyads. This interaction is mediated by the Src-homology 3 domain of Cav2 and is necessary for an effective pacing frequency‐dependent increase of the Ca2+-induced Ca2+ release mechanism in cardiomyocytes.
HostingRepository	PRIDE
AnnounceDate	2021-12-14
AnnouncementXML	Submission_2021-12-14_03:33:40.260.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021375
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katalin Barkovits
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 03:50:28	ID requested
⏵ 1	2021-12-14 03:33:40	announced

Dataset with its publication pending

submitter keyword: Cardiomyocytes, L-type voltage-gated calcium channels (LTCCs), Ca2+ influx, pull-down, dSTORM, proteomics, nano-environments of Cav2

Dr. Erick Miranda Laferte
contact affiliation	Institut für Biologische Informationsprozesse Molekular- und Zellphysiologie (IBI-1) Forschungszentrum Jülich 52425 Jülich Germany
contact email	e.miranda.laferte@fz-juelich.de
lab head
Katalin Barkovits
contact affiliation	Ruhr University Bochum
contact email	katalin.barkovits@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD021375

PRIDE project URI

• PRIDE
  1. PXD021375
     1. Label: PRIDE project
     2. Name: Interactome of the -subunit of L-type voltage-gated calcium channels in adult cardiomyocytes