PXD021374

PXD021374 is an original dataset announced via ProteomeXchange.

Title	Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
Description	Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of the ribosomal subunits, whose production and modifications are steered and regulated by numerous biogenesis factors. In this study, we analyze unperturbed native prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits by using an E. coli strain, producing the affinity tagged biogenesis factor ObgE from its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2022-11-17
AnnouncementXML	Submission_2022-11-16_23:40:43.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos HenriqueVieira e Vieira
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 03:31:47	ID requested
⏵ 1	2022-11-16 23:40:44	announced

Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, B, ü, rger J, Loerke J, Amikura K, Fl, ü, gel T, Ueda T, Selbach M, Deuerling E, Spahn CMT, Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. Mol Cell, 81(6):1200-1215.e9(2021) [pubmed]

submitter keyword: LC-MSMS,bacteria, ribosome, shotgun

MatthiasSelbach
contact affiliation	Proteome Dynamics, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Rössle-Str. 10, 13125 Berlin, Germany
contact email	matthias.selbach@mdc-berlin.de
lab head
Carlos HenriqueVieira e Vieira
contact affiliation	Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft
contact email	carlos.vieira@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD021374

PRIDE project URI

• PRIDE
  1. PXD021374
     1. Label: PRIDE project
     2. Name: Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization