PXD021373

PXD021373 is an original dataset announced via ProteomeXchange.

Title	Identification of sensitiser modified HLA-peptides presented by keratinocytes
Description	Skin sensitisation following the covalent modification of proteins by low molecular weight chemicals (haptenation) is mediated by cytotoxic T lymphocyte (CTL) recognition of human leukocyte antigen molecules (HLA) presented on the surface of almost all nucleated cells. There are three nonmutually exclusive models for how haptens mediate CTL recognition: direct stimulation by haptenated peptides; hapten modification of HLA leading to an altered HLA-peptide repertoire; or a hapten altered proteome leading to an an altered HLA-peptide repertoire. To address the mechanism in skin, we set out to utilise proteomic analysis of keratinocyte presented antigens following exposure to 2,4-dinitrochlorobenzene (DNCB). We show that following DNCB exposure, cultured keratinocytes present cysteine haptenated (dinitrophenylated) peptides in multiple HLA molecules. In addition, we find that one of the DNCB modified peptides derives from the active site of cytosolic glutathione-S transferase-ω. These results provide support for the hypothesis that a key mechanism of skin sensitisation is stimulation of CTLs by haptenated peptides.
HostingRepository	PRIDE
AnnounceDate	2021-07-01
AnnouncementXML	Submission_2021-07-01_04:24:05.526.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021373
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alistair Bailey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; reagent derivatized residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 02:32:15	ID requested
⏵ 1	2021-07-01 04:24:06	announced

Bailey A, Nicholas B, Darley R, Parkinson E, Teo Y, Aleksic M, Maxwell G, Elliott T, Ardern-Jones M, Skipp P, Characterization of the Class I MHC Peptidome Resulting From DNCB Exposure of HaCaT Cells. Toxicol Sci, 180(1):136-147(2021) [pubmed]

submitter keyword: Human, peptidome, DNCB, keratinocytes, HLA

Paul J Skipp
contact affiliation	Centre for Proteomic Research B85, Life Sciences Building University of Southampton University Road Highfield Southampton, Hants. SO17 1BJ United Kingdom
contact email	pjss@soton.ac.uk
lab head
Alistair Bailey
contact affiliation	University of Southampton
contact email	ab604@soton.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021373
     1. Label: PRIDE project
     2. Name: Identification of sensitiser modified HLA-peptides presented by keratinocytes