PXD021367

PXD021367 is an original dataset announced via ProteomeXchange.

Title	The Impact of Blood-Processing Time on the Proteome of Human Peripheral Blood Mononuclear Cells
Description	Human peripheral blood mononuclear cells (PBMC) are key to several diagnostics assays and basic science research. Blood pre-analytical variations that occur before obtaining the PBMC fraction can have a significant impact on the results of the assays, including viability, composition, integrity, and gene expression changes of immune cells. With this as motivation, we performed a quantitative shotgun proteomics analysis using Isobaric Tag for Relative and Absolute Quantitation (iTRAQ 8plex) labeling to compare PBMC obtained from fresh versus 24h-stored blood at room temperature. We identified 3,195 proteins, of which 245 were differentially abundant. Our results revealed enriched pathways in the fresh-PBMC samples related to exocytosis, localization, vesicle-mediated transport, cell activation, and secretion. In contrast, pathways related to exocytosis, neutrophil degranulation and activation, granulocyte activation, leukocyte degranulation, and myeloid leukocyte activation involved in immune response were enriched in the 24h-PBMC samples, which may indicate probable granulocyte contamination and activation due to blood storage time and temperature. Examples of upregulated proteins in the 24h-PBMC sample are: CAMP, S100A8, LTA4H, RASAL3, and S100A6, which are involved in an adaptive immune system and antimicrobial activity, proinflammatory mediation, aminopeptidase activities, and naïve T cells survival. Moreover, examples of downregulated proteins are: NDUFA5, TAGLN2, H3C1, TUBA8, and CCT2 that are related to the cytoskeleton, cell junction, mitochondrial respiratory chain. In conclusion, blood-processing time directly impacts the proteomic profile of human PBMC.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-14_00:06:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlon D M Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 01:03:43	ID requested
⏵ 1	2020-12-14 00:06:10	announced

Bonilauri B, Santos MDM, Camillo-Andrade AC, Bispo S, Nogueira FCS, Carvalho PC, Zanchin NIT, Fischer JSDG, The impact of blood-processing time on the proteome of human peripheral blood mononuclear cells. Biochim Biophys Acta Proteins Proteom, 1869(3):140581(2021) [pubmed]

submitter keyword: Blood, PBMC, iTRAQ, Proteomics, Immunology.

Paulo Costa Carvalho
contact affiliation	Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz–PR, Brazil.
contact email	paulo@pcarvalho.com
lab head
Marlon D M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021367
     1. Label: PRIDE project
     2. Name: The Impact of Blood-Processing Time on the Proteome of Human Peripheral Blood Mononuclear Cells