PXD021353

PXD021353 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison between two Salmonella serovars with differential pathogenicity in humans.
Description	Salmonella infections are among the most common foodborne diseases worldwide. The Enteritidis and Dublin serovars of Salmonella enterica are closely related yet they differ significantly in pathogenicity and epidemiology. Enteritidis is a broad-host-range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease. The aim of this work was to elucidate the molecular factors responsible for the differential pathogenic behavior between both serovars. We performed a quantitative proteomic comparative analysis between one clinical isolate of each serovar grown in vitro under gut mimicking conditions (GMC). Compared to S. Enteritidis, the S. Dublin proteome was enriched in proteins linked to response to several stress conditions, such as those encountered during host infection, as well as to virulence. The S. Enteritidis proteome contained proteins related to central anaerobic metabolism pathways that were undetected in S. Dublin. Similar differences were also found at the transcriptional level, as mRNA levels correlated with proteomic results for 17 of the 20 genes tested in 4 natural isolates of each serovar grown in GMC. This work reveals proteomic differences between two Salmonella serovars with markedly different invasive and host-range characteristics, grown in an infection relevant condition, which were not evident in previous comparative genomic analyses.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_09:03:00.643.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adriana Martínez
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Enteritidis; NCBI TaxID: 149539; scientific name: Salmonella enterica subsp. enterica serovar Dublin; NCBI TaxID: 98360;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-08 01:04:36	ID requested
⏵ 1	2021-09-09 09:03:01	announced

Martinez-Sanguin, é AY, D'Alessandro B, Langleib M, Traglia GM, M, ó, naco A, Dur, á, n R, Chabalgoity JA, Betancor L, Yim L, Salmonella enterica Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism. Infect Immun, 89(3):(2021) [pubmed]

submitter keyword: “Salmonella enterica serovar Dublin”, “Salmonella Enteritidis”, “gut mimicking conditions”

Lucia Nan-Mei Yim Leone
contact affiliation	Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina,Universidad de la República, Montevideo, Uruguay
contact email	lyim@higiene.edu.uy
lab head
Adriana Martínez
contact affiliation	facultad de Medicina, UDELAR
contact email	amartinez@higiene.edu.uy
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021353

PRIDE project URI

• PRIDE
  1. PXD021353
     1. Label: PRIDE project
     2. Name: Proteomic comparison between two Salmonella serovars with differential pathogenicity in humans.