PXD021351

PXD021351 is an original dataset announced via ProteomeXchange.

Title	Whole-cell and plasma membrane proteomics of herpes simplex virus-1 infection reveals cell surface remodelling via pUL56-mediated GOPC degradation
Description	Herpesviruses are ubiquitous in the human population and they extensively remodel the cellular environment during infection. Multiplexed quantitative proteomic analysis over the time-course of herpes simplex virus (HSV)-1 infection was used to characterize changes in the host-cell proteome and the kinetics of viral protein production. Several host-cell proteins are targeted for rapid degradation by HSV-1, including the cellular trafficking factor GOPC. We show that the poorly-characterized HSV-1 pUL56 directly binds GOPC, stimulating its ubiquitination and proteasomal degradation. Plasma membrane profiling revealed that pUL56 mediates specific changes to the cell surface proteome of infected cells, including loss of IL18 receptor and Toll-like receptor 2, and that cell surface expression of Toll-like receptor 2 is GOPC-dependent. Our study provides significant resources for future investigation of HSV-host interactions and highlights an unanticipated and efficient mechanism whereby a single virus protein targets a cellular trafficking factor to modify the surface of infected cells.
HostingRepository	PRIDE
AnnounceDate	2020-10-15
AnnouncementXML	Submission_2020-10-15_00:10:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leah Hunter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human alphaherpesvirus 1; NCBI TaxID: 10298;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; biotinylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-07 23:03:19	ID requested
⏵ 1	2020-10-15 00:10:09	announced

Dataset with its publication pending

submitter keyword: Herpesvirus, Virus host interaction, Immune evasion, Membrane trafficking, Proteasomal degradation, Quantitative proteomics, Uncharacterized ORF

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact email	mpw1001@cam.ac.uk
lab head
Leah Hunter
contact affiliation	University of Cambridge
contact email	lmh87@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021351
     1. Label: PRIDE project
     2. Name: Whole-cell and plasma membrane proteomics of herpes simplex virus-1 infection reveals cell surface remodelling via pUL56-mediated GOPC degradation