PXD021349

PXD021349 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomic analysis of thermally challenged Caribbean reef corals
Description	In this work, I took a proteome profiling approach to characterize the cellular biology of 16 coral samples from a laboratory-based thermal stress experiment. The specific goal of this work was to uncover proteins involved in the high-temperature response of the Caribbean reef coral Orbicella faveolata. Coral colonies from two inshore (Cheeca Rocks [UKI] & The Rocks [UKI2]) and one offshore reef (Little Conch [UKO2]) were cored in situ, with cores later brought to the “Experimental Reef Laboratory” (PI Ian Enochs, NOAA) housed at the University of Miami’s Rosenstiel School of Marine and Atmospheric Sciences (RSMAS). After laboratory acclimation, the samples were exposed to either 33°C for 5 days or 32°C days for 31 days, with control corals maintained at 30°C. Proteins were extracted from a subset of samples representing multiple coral genotypes exposed to the experimental treatment conditions for 5 or 31 days and sequenced with nano-liquid chromatography followed by mass spectrometry. In this submission, I have uploaded a draft of the manuscript describing these data (“Mayfield et al., in prep;” with the formal publication likely to be published in 2021), 16 RAW files generated by the mass spectrometer, 16 MZML (open-access mass spectrometry mass peak data) files, 16 MZID (open-access mass spectrometry results) files, and a tab-delineated file that describes which samples were analyzed ("Mayfield et al. online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield et al. (in prep.) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against both conceptually translated Orbicella faveolata and Symbiodiniaceae dinoflagellate transcriptomes (described in Aguilar et al. in review); both fasta files have been uploaded.
HostingRepository	MassIVE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_18:40:24.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anderson B Mayfield
SpeciesList	scientific name: Orbicella faveolata; NCBI TaxID: 48498; scientific name: Breviolum sp.; NCBI TaxID: 2499526; scientific name: Durusdinium sp.; NCBI TaxID: 2486700;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-07 18:10:14	ID requested
⏵ 1	2020-09-07 18:40:24	announced

no publication

submitter keyword: coral reefs, dinoflagellates

Anderson Mayfield
contact affiliation	NOAA
contact email	anderson.mayfield@noaa.gov
lab head
Anderson B Mayfield
contact affiliation	NOAA
contact email	anderson.mayfield@noaa.gov
dataset submitter

MassIVE dataset URI

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