PXD021309

PXD021309 is an original dataset announced via ProteomeXchange.

Title	CD209L/L-SIGN N-Glycosylation and N-Glycosylation Site Occupancy
Description	CD209L is a membrane glycoprotein with known glycan-binding properties and it also contains 2 N-glycosylation sequons at sites N92 and N361. Treatment with PNGase F in the presence of H218O, which removes N-linked glycans and isotopically labels the formerly-glycosylated site, confirmed that both unmodified and formerly-glycosylated versions of the peptide spanning the N92 N-glycosylated sequon were present. This suggests that a portion of CD209L protein is N-glycosylated at site N92. nUPLC-MS/MS analyses of CD209L digests enabled detection of a glycopeptide consistent with high-mannose type N-linked glycosylation.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:58:04.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Chandler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-04 02:50:36	ID requested
⏵ 1	2021-09-09 02:58:05	announced

Amraei R, Yin W, Napoleon MA, Suder EL, Berrigan J, Zhao Q, Olejnik J, Chandler KB, Xia C, Feldman J, Hauser BM, Caradonna TM, Schmidt AG, Gummuluru S, M, ü, hlberger E, Chitalia V, Costello CE, Rahimi N, CD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV-2. ACS Cent Sci, 7(7):1156-1165(2021) [pubmed]

submitter keyword: Human CD209L, DC-SIGN, N-Glycosylation

Catherine E Costello
contact affiliation	William Fairfield Warren Distinguished Professor Depts. Of Biochemistry, Biophysics and Chemistry Boston University School of Medicine Boston, MA, USA
contact email	cecmsms@bu.edu
lab head
Kevin Chandler
contact affiliation	Boston University School of Medicine
contact email	chandler64@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021309

PRIDE project URI

• PRIDE
  1. PXD021309
     1. Label: PRIDE project
     2. Name: CD209L/L-SIGN N-Glycosylation and N-Glycosylation Site Occupancy