PXD021299

PXD021299 is an original dataset announced via ProteomeXchange.

Title	The temperature-regulation of Pseudomonas aeruginosa cmaX-cfrX-cmpX operon reveals an intriguing molecular network involving the sigma factors AlgU and SigX
Description	Pseudomonas aeruginosa is a highly adaptable Gram-negative opportunistic pathogen, notablydue to its large number of transcription regulators. The extracytoplasmic sigma factor (ECF sigma AlgU, responsible for alginate biosynthesis, is also involved in responses to cell wall stress and heat shock via the RpoH alternative sigma factor. The SigX ECF sigma emerged as a major regulator involved in the envelope stress response via membrane remodelling, virulence and biofilm formation. However, their functional interactions to coordinate the envelope homeostasis in response to environmental variations remain to be determined. The regulation of the putative cmaX-cfrX-cmpX operon located directly upstream sigX was investigated by applying sudden temperature shifts from 37°C. We identified a SigX- and an AlgU- dependent promoter region upstream of cfrX and cmaX, respectively. We show that cmaX expression is increased upon heat shock through an AlgU-dependent but RpoH independent mechanism. In addition, the ECF sigma SigX is activated in response to valinomycin, an agent altering the membrane structure, and up-regulates cfrX-cmpX transcription in response to cold shock. Altogether, these data provide new insights into the regulation exerted by SigX and networks that are involved in maintaining envelope homeostasis.
HostingRepository	PRIDE
AnnounceDate	2020-10-05
AnnouncementXML	Submission_2020-11-17_08:49:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emeline Bouffartigues
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-04 02:49:30	ID requested
1	2020-10-04 23:22:28	announced
⏵ 2	2020-11-17 08:49:04	announced	2020-11-17: Updated publication reference for PubMed record(s): 33193206.

Bouffartigues E, Si Hadj Mohand I, Maillot O, Tortuel D, Omnes J, David A, Tahrioui A, Duchesne R, Azuama CO, Nusser M, Brenner-Weiss G, Bazire A, Connil N, Orange N, Feuilloley MGJ, Lesouhaitier O, Dufour A, Cornelis P, Chevalier S, Operon Reveals an Intriguing Molecular Network Involving the Sigma Factors AlgU and SigX. Front Microbiol, 11():579495(2020) [pubmed]

submitter keyword: Pseudomonas aeruginosa, cold shock, 2D-electrophoresis

Prof Marc Feuilloley
contact affiliation	Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM) EA 4312, Normandie Université, Université deRouen Normandie, Centre de Sécurité Sanitaire de Normandie, Evreux, France.
contact email	marc.feuilloley@univ-rouen.fr
lab head
Emeline Bouffartigues
contact affiliation	LMSM EA4312 University of Rouen
contact email	emeline.bouffartigues@univ-rouen.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021299
     1. Label: PRIDE project
     2. Name: The temperature-regulation of Pseudomonas aeruginosa cmaX-cfrX-cmpX operon reveals an intriguing molecular network involving the sigma factors AlgU and SigX