PXD021293

PXD021293 is an original dataset announced via ProteomeXchange.

Title	ITRAQ proteomic analysis of yellow and black skin in Jinbian carp (Cyprinus carpio)
Description	Colors are important phenotypic traits for fitness under natural conditions in vertebrates. Previous studies have reported several functional genes and genetic variations of pigmentation, but the formation mechanisms of various skin coloration were ambiguous in fish. Jinbian carp, a common carp variant, displays two colors (yellow and black) in the skin, so it is a good model for investigating the genetic basis of pigmentation. In the present study, using the Jinbian carp as model, ITRAQ proteomics analysis was performed for yellow and black skin, respectively. The results showed that 467 differentially expressed proteins (DEPs) were identified between the yellow skin and the black skin. Similar to mammals, the up-regulated DEPs in black skin included UV excision repair protein RAD23 homolog A (Rad23a), melanoregulin (mreg), 5,6-dihydroxyindole-2-carboxylic acid oxidase5 (tyrp1) and melanocyte protein PMEL (PMEL), which were mainly grouped into melanogenesis pathway. However, several up-regulated DEPs in yellow skin were mainly enriched in nucleotide metabolism, such as GTPase IMAP family member 5 (GIMAP5), AMP deaminase 1 (AMPD1), adenosylhomocysteinase b (ahcy-b), and pyruvate kinase (PKM). In summary, several candidate proteins and their enrichment pathways for colour variation in Jinbian carp were identified, which may be responsible for the formation of different colorations.
HostingRepository	iProX
AnnounceDate	2020-09-04
AnnouncementXML	Submission_2023-03-01_23:08:16.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guangfu Hu
SpeciesList	scientific name: Cyprinus carpio haematopterus; NCBI TaxID: 1045269;
ModificationList	No PTMs are included in the dataset
Instrument	iTRAQ quantitation analysis

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-03 22:29:44	ID requested
1	2020-09-03 22:31:16	announced
⏵ 2	2023-03-01 23:08:16	announced	2023-03-02: Update publication information.

Ye X, Zhou L, Jia J, Wei L, Wen Y, Yan X, Huang J, Gan B, Liu K, Lv Y, Hu G, ). Life (Basel), 10(10):(2020) [pubmed]

submitter keyword: pigmentation, ITRAQ, Jinbian carp, melanin, pteridine

Guangfu Hu
contact affiliation	Huazhong Agricultural University
contact email	huguangfu@mail.hzau.edu.cn
lab head
Guangfu Hu
contact affiliation	Huazhong Agricultural University
contact email	huguangfu@mail.hzau.edu.cn
dataset submitter

iProX dataset URI