PXD021252 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A proteomic view of cellular responses to copper ion in two different modes |
Description | Copper is both an essential metal for life, but is also toxic at high concentrations. In mammalian cells, two copper transporters are known, one localized at the plasma membrane (CTR1) and one localized in late endosomes-lysosomes (CTR2). In order to gain insights on the possible influence of the importing pathway on cellular responses to copper, two copper challenges were compared, one with copper ion, which is likely to use preferentially CTR1, and one with a copper-polyacrylate complex, which will likely to be internalized via the endosomal pathway and therefore use preferentially CTR2. A model system consisting in the J774A1 mouse macrophage system, with a strong endosomal/lysosomal pathway, was used. In order to gain wide insights into the cellular responses to copper, a two-dimensional gel-based proteomic approach was used. The proteomic results were validated by targeted experiments, and showed differential effects of the import mode on general cellular physiology parameters such as the reduced glutathione content, the actin cytoskeleton or the mitochondrial potential, but also on more specialized macrophage functions such as phagocytosis or inflammatory responses. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-08 |
AnnouncementXML | Submission_2024-07-08_06:53:52.887.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hélène Diemer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600; Synapt MS; maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-01 04:15:21 | ID requested | |
⏵ 1 | 2024-07-08 06:53:53 | announced | |
Publication List
Keyword List
submitter keyword: Macrophage, 2D gel proteomic, Mouse,Copper, LC-MS/MS |
Contact List
Sarah CIANFERANI |
contact affiliation | Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg Cedex 2, France |
contact email | sarah.cianferani@unistra.fr |
lab head | |
Hélène Diemer |
contact affiliation | LSMBO |
contact email | hdiemer@unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021252
- Label: PRIDE project
- Name: A proteomic view of cellular responses to copper ion in two different modes