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PXD021252

PXD021252 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomic view of cellular responses to copper ion in two different modes
DescriptionCopper is both an essential metal for life, but is also toxic at high concentrations. In mammalian cells, two copper transporters are known, one localized at the plasma membrane (CTR1) and one localized in late endosomes-lysosomes (CTR2). In order to gain insights on the possible influence of the importing pathway on cellular responses to copper, two copper challenges were compared, one with copper ion, which is likely to use preferentially CTR1, and one with a copper-polyacrylate complex, which will likely to be internalized via the endosomal pathway and therefore use preferentially CTR2. A model system consisting in the J774A1 mouse macrophage system, with a strong endosomal/lysosomal pathway, was used. In order to gain wide insights into the cellular responses to copper, a two-dimensional gel-based proteomic approach was used. The proteomic results were validated by targeted experiments, and showed differential effects of the import mode on general cellular physiology parameters such as the reduced glutathione content, the actin cytoskeleton or the mitochondrial potential, but also on more specialized macrophage functions such as phagocytosis or inflammatory responses.
HostingRepositoryPRIDE
AnnounceDate2024-07-08
AnnouncementXMLSubmission_2024-07-08_06:53:52.887.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHélène Diemer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600; Synapt MS; maXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-01 04:15:21ID requested
12024-07-08 06:53:53announced
Publication List
10.1016/J.JPROT.2021.104178;
Keyword List
submitter keyword: Macrophage, 2D gel proteomic, Mouse,Copper, LC-MS/MS
Contact List
Sarah CIANFERANI
contact affiliationLaboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg Cedex 2, France
contact emailsarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliationLSMBO
contact emailhdiemer@unistra.fr
dataset submitter
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Dataset FTP location
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