PXD021252

PXD021252 is an original dataset announced via ProteomeXchange.

Title	A proteomic view of cellular responses to copper ion in two different modes
Description	Copper is both an essential metal for life, but is also toxic at high concentrations. In mammalian cells, two copper transporters are known, one localized at the plasma membrane (CTR1) and one localized in late endosomes-lysosomes (CTR2). In order to gain insights on the possible influence of the importing pathway on cellular responses to copper, two copper challenges were compared, one with copper ion, which is likely to use preferentially CTR1, and one with a copper-polyacrylate complex, which will likely to be internalized via the endosomal pathway and therefore use preferentially CTR2. A model system consisting in the J774A1 mouse macrophage system, with a strong endosomal/lysosomal pathway, was used. In order to gain wide insights into the cellular responses to copper, a two-dimensional gel-based proteomic approach was used. The proteomic results were validated by targeted experiments, and showed differential effects of the import mode on general cellular physiology parameters such as the reduced glutathione content, the actin cytoskeleton or the mitochondrial potential, but also on more specialized macrophage functions such as phagocytosis or inflammatory responses.
HostingRepository	PRIDE
AnnounceDate	2024-07-08
AnnouncementXML	Submission_2024-07-08_06:53:52.887.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600; Synapt MS; maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-01 04:15:21	ID requested
⏵ 1	2024-07-08 06:53:53	announced

10.1016/J.JPROT.2021.104178;

submitter keyword: Macrophage, 2D gel proteomic, Mouse,Copper, LC-MS/MS

Sarah CIANFERANI
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg Cedex 2, France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021252
     1. Label: PRIDE project
     2. Name: A proteomic view of cellular responses to copper ion in two different modes