PXD021233

PXD021233 is an original dataset announced via ProteomeXchange.

Title	CD4+ T cell specific proteomic pathways identified in progression of hypertension across postmenopausal transition
Description	Menopause is associated with an increase in the prevalence and severity of hypertension in women. Although premenopausal females are protected against T cell-dependent immune activation and development of angiotensin II (Ang II) hypertension, this protection is lost in postmenopausal females. Therefore, the current study hypothesized that specific CD4+ T cell pathways are regulated by sex hormones and Ang II to mediate progression from premenopausal protection to postmenopausal hypertension. Menopause was induced in C57BL/6 mice via repeated 4-vinylcyclohexene diepoxide (VCD) injections, while premenopausal females received sesame oil vehicle. A subset of premenopausal mice and all menopausal mice were infused with Ang II for 14 days (Control, Ang II, Meno/Ang II). Proteomic and phosphoproteomic profiles of CD4+ T cells isolated from spleens were examined.Ang II markedly increased CD4+ T cell protein abundance and phosphorylation associated with DNA and histone methylation in both premenopausal and postmenopausal females. Compared to premenopausal T cells, Ang II infusion in menopausal mice increased T cell phosphorylation of MP2K2, an upstream regulator of ERK, and was associated with upregulated phosphorylation at ERK targeted sites. Additionally, Ang II infusion in menopausal mice decreased T cell phosphorylation of TLN1, a key regulator of IL-2R and FOXP3 expression. These findings identify novel, distinct intracellular T cell pathways that influence T cell-mediated inflammation during postmenopausal hypertension.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:36:58.353.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021233
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Langlais
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-01 00:34:19	ID requested
⏵ 1	2021-09-09 10:36:59	announced

Uhlorn JA, Husband NA, Romero-Aleshire MJ, Moffett C, Lindsey ML, Langlais PR, Brooks HL, T Cell-Specific Proteomic Pathways Identified in Progression of Hypertension Across Postmenopausal Transition. J Am Heart Assoc, 10(2):e018038(2021) [pubmed]

submitter keyword: postmenopausal hypertension CD4+ T cell

Heddwen Brooks
contact affiliation	Professor, Depts of Physiology, Pharmacology, Nephrology/Medicine, Member, BIO5/Arizona Center on Aging/Sarver Heart Center, University of Arizona College of Medicine
contact email	brooksh@arizona.edu
lab head
Paul Langlais
contact affiliation	University of Arizona
contact email	langlais@deptofmed.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021233
     1. Label: PRIDE project
     2. Name: CD4+ T cell specific proteomic pathways identified in progression of hypertension across postmenopausal transition