PXD021227

PXD021227 is an original dataset announced via ProteomeXchange.

Title	CSR-1A post-translational modification
Description	CSR-1 is an essential Argonaute protein that binds to a subclass of 22G-RNAs targeting most germline-expressed genes. Here we show that the two isoforms of CSR-1 have distinct expression patterns; CSR-1B is ubiquitously expressed throughout the germline and during all stages of development while CSR-1A expression is restricted to germ cells undergoing spermatogenesis. Furthermore, CSR-1A associates preferentially with 22G-RNAs mapping to spermatogenesis-specific genes whereas CSR-1B-bound small RNAs map predominantly to oogenesis-specific genes. Interestingly, the exon unique to CSR-1A contains multiple dimethylarginine modifications, which are necessary for the preferential binding of CSR-1A to spermatogenesis-specific 22G-RNAs. Thus, we have discovered a regulatory mechanism for C. elegans Argonaute proteins that allows for specificity of small RNA binding between similar Argonaute proteins with overlapping temporal and spatial localization.
HostingRepository	PRIDE
AnnounceDate	2021-07-20
AnnouncementXML	Submission_2021-07-20_02:35:45.357.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021227
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Carolyn Phillips
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	methylated residue; dimethylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 22:07:19	ID requested
1	2021-07-20 02:35:05	announced
⏵ 2	2021-07-20 02:35:46	announced	2021-07-20: Updated publication reference for PubMed record(s): 34244496.

Nguyen DAH, Phillips CM, Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1. Nat Commun, 12(1):4212(2021) [pubmed]

submitter keyword: C. elegans, CSR-1A, dimethylation

Carolyn M. Phillips
contact affiliation	Department of Biological Sciences, University of Southern California, Los Angeles CA
contact email	cphil@usc.edu
lab head
Carolyn Phillips
contact affiliation	University of Southern California
contact email	cphil@usc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021227
     1. Label: PRIDE project
     2. Name: CSR-1A post-translational modification