PXD021221

PXD021221 is an original dataset announced via ProteomeXchange.

Title	Proteomics analyses of immature (21 day post infection) Fasciola hepatica
Description	The major pathogenesis associated with Fasciola hepatica infection results from the extensive tissue damage caused by the tunnelling and feeding activity of immature flukes during their migration, growth and development in the liver. This is compounded by the pathology caused by host innate and adaptive immune responses that struggle to repair this damage. Complementary transcriptomic and proteomic approaches defined the F. hepatica factors associated with their migration in the liver, and the resulting immune-pathogenesis. The liver-stage parasites display different secretome profiles, reflecting their distinct niche within the host, and supports the view that cathepsin peptidases, cathepsin peptidase inhibitors, saposins and leucine aminopeptidases play a central role in the parasite’s destructive migration, digest of host tissue and blood. Immature flukes are also primed for countering immune attack by secreting immunomodulating fatty acid binding proteins (FABP) and helminth defense molecules (FhHDM). The migration of immature F. hepatica parasites within the liver is associated with an increase in protein production, expression of signalling pathways and neoblast proliferation that drive their rapid growth and development. The secretion of a defined set of molecules, particularly cathepsin L peptidases, peptidase-inhibitors, saponins, immune-regulators and anti-oxidants allow the parasite to negotiate the liver micro-environment, immune attack and increasing levels of oxidative stress. This data contributes to the growing F. hepatica -omics information that can be exploited to understand parasite development more fully and for the design of novel control strategies to prevent host liver tissue destruction and pathology.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_09:19:17.671.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Krystyna Cwiklinski
SpeciesList	scientific name: Fasciola hepatica; NCBI TaxID: 6192;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 04:04:13	ID requested
⏵ 1	2021-09-09 09:19:18	announced

Cwiklinski K, Robinson MW, Donnelly S, Dalton JP, Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver. BMC Genomics, 22(1):46(2021) [pubmed]

submitter keyword: Fasciola hepatica, somatic proteome, secretome, LC-MS-MS, liver, growth

John P Dalton
contact affiliation	Molecular Parasitology Lab (MPL),Orbsen Building, School of Natural Sciences/Ryan Institute National University of Ireland, Galway, Ireland
contact email	johnpius.dalton@nuigalway.ie
lab head
Krystyna Cwiklinski
contact affiliation	NUI Galway
contact email	krystyna.cwiklinski@nuigalway.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021221

PRIDE project URI

• PRIDE
  1. PXD021221
     1. Label: PRIDE project
     2. Name: Proteomics analyses of immature (21 day post infection) Fasciola hepatica