PXD021216

PXD021216 is an original dataset announced via ProteomeXchange.

Title	Alkaloids modify the poison frog proteome
Description	Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (Oophaga sylvatica) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within four days, and dietary alkaloid exposure modified protein abundance in the intestines, liver, and skin. Many proteins that increased in abundance with toxin accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with toxin accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues
HostingRepository	PRIDE
AnnounceDate	2021-01-25
AnnouncementXML	Submission_2021-01-24_23:00:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021216
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeremy OConnell
SpeciesList	scientific name: Oophaga sylvatica; NCBI TaxID: 152496;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 01:54:12	ID requested
⏵ 1	2021-01-24 23:00:30	announced

O'Connell LA, O'Connell JD, Paulo JA, Trauger SA, Gygi SP, Murray AW, Rapid toxin sequestration modifies poison frog physiology. J Exp Biol, 224(Pt 3):(2021) [pubmed]

submitter keyword: Poison Dart Frog,gut, liver, skin, toxin, saxiphilin, complement system, cytochrome P450s

Lauren A. O’Connell
contact affiliation	Department of Biology Stanford University 371 Jane Stanford Way Stanford, CA 94305
contact email	loconnel@stanford.edu
lab head
Jeremy OConnell
contact affiliation	Harvard Medical School
contact email	theoconnell@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021216
     1. Label: PRIDE project
     2. Name: Alkaloids modify the poison frog proteome